Cellular dynamics in the draining lymph nodes during sensitization and elicitation phases of contact hypersensitivity
| Autor: | Jeppe Madura Larsen, Martin Weiss Nielsen, Marina Rode von Essen, Ann Kathrine Hansen, Charlotte M. Bonefeld, Carsten Geisler, Lone Skov, Lasse Boding |
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| Rok vydání: | 2007 |
| Předmět: |
Cell type
T-Lymphocytes Lymphocyte Dermatology Lymphocyte Activation Antibodies Flow cytometry Mice Immune system medicine Animals Immunology and Allergy Cytotoxic T cell Lymph node biology medicine.diagnostic_test business.industry Oxazolone Allergens Flow Cytometry Mice Inbred C57BL medicine.anatomical_structure Dermatitis Allergic Contact Immunology biology.protein Dinitrofluorobenzene Female Lymph Nodes Lymph Antibody business |
| Zdroj: | Contact Dermatitis. 57:300-308 |
| ISSN: | 1600-0536 0105-1873 |
| DOI: | 10.1111/j.1600-0536.2007.01230.x |
| Popis: | Background: The different role of various immunological effector cells in contact hypersensitivity (CHS) is receiving increased attention. During the past decade, the involvement of different cell types in CHS has been investigated by the use of antibody-induced depletion of specific subtypes of immunological cells and by studying knockout mice lacking one or more of these immunological cell populations. Objectives: To develop a method for studying the collective cellular dynamics of immune cells in the draining lymph nodes during CHS in intact animals. Patients/Methods: Mice were sensitized and/or challenged with 2,4-dinitrofluorobenzene or oxazolone. Using multi-parameter flow cytometry we determined the proliferation, activation state, and absolute number of helper T cells, cytotoxic T cells, B cells, and natural killer cells in the draining lymph nodes. Results: The presented method can be applied to evaluate the effect of different contact allergens on various cell populations of the immune system. Conclusions: Our study support recent findings that several cell types seem to be involved in CHS. |
| Databáze: | OpenAIRE |
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