Hypoxia Induces Expression and Activation of AMPK in Rat Dental Pulp Cells
| Autor: | Kazumasa Ohta, Y. Fukuyama, Harutoshi Kizaki, Rintarou Okoshi, Masataka Suehara, Kan-Ichi Nakagawa |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
0301 basic medicine
Blotting Western Oxidative phosphorylation AMP-Activated Protein Kinases Protein Serine-Threonine Kinases Statistics Nonparametric Cell Line 03 medical and health sciences Enzyme activator 0302 clinical medicine AMP-activated protein kinase Multienzyme Complexes medicine Animals RNA Small Interfering Hypoxia Protein kinase A General Dentistry Dental Pulp Cell Proliferation Analysis of Variance biology Reverse Transcriptase Polymerase Chain Reaction Chemistry Cell growth AMPK 030206 dentistry Hypoxia (medical) Hypoxia-Inducible Factor 1 alpha Subunit Rats Cell biology Enzyme Activation Isoenzymes 030104 developmental biology Cell culture Enzyme Induction biology.protein medicine.symptom |
| Zdroj: | Journal of Dental Research. 86:903-907 |
| ISSN: | 1544-0591 0022-0345 |
| DOI: | 10.1177/154405910708600919 |
| Popis: | AMP-activated protein kinase (AMPK) is a stress-responsive enzyme involved in cell adaptation to an energy crisis. We hypothesized that hypoxia suppresses oxidative phosphorylation and ATP production, resulting in AMPK activation to protect cells. We investigated the effects of hypoxia on cell proliferation, the expression of AMPK and hypoxia-inducible factor 1alpha (HIF-1alpha), the activation of AMPK, and the relationship between AMPK and HIF-1alpha expression in rat dental pulp RPC-C2A cells. AMPK in the cells was composed of catalytic alpha1, and regulatory beta1 and gamma1 subunit isoforms. Cell proliferation was initially suppressed under hypoxia, but it increased thereafter, together with an increase in the expression of AMPK and HIF-1alpha, and the activation of AMPK. Down-regulation of AMPKalpha1 by siRNA inhibited cell proliferation under both normoxia and hypoxia, revealing that AMPK induction and activation were required for cell proliferation, although HIF-1alpha expression under hypoxia was not affected. |
| Databáze: | OpenAIRE |
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