Genotyping and characterization of the geographical distribution of tick-borne encephalitis virus variants with a set of molecular probes
| Autor: | E.K. Doroshchenko, Alexander Plyusnin, O. V. Lisak, Demina Tv, M. M. Verkhozina, Tkachev Se, Yu. P. Dzhioev, I.V. Kozlova, Zlobin Vi |
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| Rok vydání: | 2010 |
| Předmět: |
Genotype
030231 tropical medicine Biology Encephalitis Viruses Tick-Borne Russia 03 medical and health sciences 0302 clinical medicine Virology Genetic variation Encephalitis Viruses Humans Genetic variability Genotyping 030304 developmental biology Genetics 0303 health sciences Polymorphism Genetic Nucleic Acid Hybridization biology.organism_classification 3. Good health Tick-borne encephalitis virus Flavivirus Infectious Diseases Molecular Probes Genetic structure RNA Viral |
| Zdroj: | Journal of Medical Virology. 82:965-976 |
| ISSN: | 1096-9071 0146-6615 |
| Popis: | A panel of deoxyoligonucleotide probes for studying the genetic variability and genotyping of Tick-borne encephalitis virus (TBEV) strains by molecular hybridization of nucleic acids (MHNA) was created. This panel allows to estimate the genetic structure of individual TBEV strains, as the targets for probes are both variable and genotype (subtype)-specific sequences of all TBEV genes. With the help of this panel using the method of molecular hybridization of nucleic acids 268 archived TBEV isolates were investigated and the distribution of its genotypes and subgenotypes of genotype 3 was made more precise in the territory of Eurasia. The conclusion made earlier has confirmed that five genotypes of TBEV co-circulate in Eastern Siberia. It is generally recognized that the Far Eastern (TBE-FE), European (TBE-Eu), and Siberian (TBE-Sib) genotypes are widespread and epidemiologically important. The fourth genotype is presented by only one isolate, TBE178-79, originated from Irkutsk region, Russia. The fifth genotype includes 10 isolates, 1 of them, TBE886-84, was found earlier and recognized as unique [Zlobin et al. (2001b): Vopr Virusol 1:12-16 (Russian)]. |
| Databáze: | OpenAIRE |
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