Plant Growth, Antibiotic Uptake, and Prevalence of Antibiotic Resistance in an Endophytic System of Pakchoi under Antibiotic Exposure
| Autor: | Xinxin Yang, Xunan Li, Linqian Bi, Qingxiang Yang, Mingming Zhou, Hao Zhang, Linlin Sun, Rongzhen Deng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Plant growth antibiotics pakchoi endophytic bacteria antibiotic-resistant genes hydroponic cultivation medicine.drug_class Tetracycline Health Toxicology and Mutagenesis 030106 microbiology Antibiotics Brassica lcsh:Medicine 010501 environmental sciences 01 natural sciences Article Microbiology 03 medical and health sciences Minimum inhibitory concentration Antibiotic resistance Drug Resistance Multiple Bacterial Vegetables medicine Endophytes Prevalence Humans 0105 earth and related environmental sciences biology Sulfamethoxazole lcsh:R Public Health Environmental and Occupational Health Drug Resistance Microbial Contamination biology.organism_classification Anti-Bacterial Agents medicine.drug |
| Zdroj: | International Journal of Environmental Research and Public Health; Volume 14; Issue 11; Pages: 1336 International Journal of Environmental Research and Public Health International Journal of Environmental Research and Public Health, Vol 14, Iss 11, p 1336 (2017) |
| ISSN: | 1660-4601 |
| DOI: | 10.3390/ijerph14111336 |
| Popis: | Antibiotic contamination in agroecosystems may cause serious problems, such as the proliferation of various antibiotic resistant bacteria and the spreading of antibiotic resistance genes (ARGs) in the environment or even to human beings. However, it is unclear whether environmental antibiotics, antibiotic resistant bacteria, and ARGs can directly enter into, or occur in, the endophytic systems of plants exposed to pollutants. In this study, a hydroponic experiment exposing pakchoi (Brassica chinensis L.) to tetracycline, cephalexin, and sulfamethoxazole at 50% minimum inhibitory concentration (MIC) levels and MIC levels, respectively, was conducted to explore plant growth, antibiotic uptake, and the development of antibiotic resistance in endophytic systems. The three antibiotics promoted pakchoi growth at 50% MIC values. Target antibiotics at concentrations ranging from 6.9 to 48.1 µg·kg−1 were detected in the treated vegetables. Additionally, the rates of antibiotic-resistant endophytic bacteria to total cultivable endophytic bacteria significantly increased as the antibiotics accumulated in the plants. The detection and quantification of ARGs indicated that four types, tetX, blaCTX-M, and sul1 and sul2, which correspond to tetracycline, cephalexin, and sulfamethoxazole resistance, respectively, were present in the pakchoi endophytic system and increased with the antibiotic concentrations. The results highlight a potential risk of the development and spread of antibiotic resistance in vegetable endophytic systems. |
| Databáze: | OpenAIRE |
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