Imaging plasma membrane phase behaviour in live cells using a thiophene-based molecular rotor
| Autor: | Douglas Wylie, Ian R. Gould, Callum J. Dickson, Phoom Chairatana, Harry L. Anderson, Marina K. Kuimova, Michael R. Dent, Ismael López-Duarte, Aurimas Vyšniauskas, Nicholas J. Brooks |
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| Přispěvatelé: | Engineering & Physical Science Research Council (EPSRC), Imperial College Trust |
| Rok vydání: | 2016 |
| Předmět: |
Fluorophore
Cell Survival Lipid Bilayers Molecular Conformation Thiophenes Molecular Dynamics Simulation 010402 general chemistry 01 natural sciences Catalysis Cell Line Microviscosity chemistry.chemical_compound Viscosity Phase (matter) Materials Chemistry Thiophene Humans Moiety Organic chemistry Lipid bilayer Mechanical Phenomena 010405 organic chemistry Cell Membrane Organic Chemistry Metals and Alloys General Chemistry Molecular Imaging 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Membrane chemistry Ceramics and Composites Biophysics 03 Chemical Sciences |
| Zdroj: | Chemical Communications. 52:13269-13272 |
| ISSN: | 1364-548X 1359-7345 |
| DOI: | 10.1039/c6cc05954f |
| Popis: | Molecular rotors have emerged as versatile probes of microscopic viscosity in lipid bilayers, although it has proved difficult to find probes that stain both phases equally in phase-separated bilayers. Here, we investigate the use of a membrane-targeting viscosity-sensitive fluorophore based on a thiophene moiety with equal affinity for ordered and disordered lipid domains, to probe ordering and viscosity within artificial lipid bilayers and live cell plasma membranes. |
| Databáze: | OpenAIRE |
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