Long Non-coding RNA LINC01234 Regulates Proliferation, Invasion and Apoptosis in Esophageal Cancer Cells
| Autor: | Yangjunqi Wang, Muhammad Ali Shahzad, Jintao Li, Minglian Wang, Sara Khodahemmati, Maliha Ghaffar, Changshuo Li, Yi Zeng, Su Chen |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gene knockdown Cell growth flow cytometry LINC01234 Cell migration Esophageal cancer Biology invasion medicine.disease Long non-coding RNA 03 medical and health sciences cell proliferation 030104 developmental biology 0302 clinical medicine Oncology Gentamicin protection assay Cell culture Apoptosis 030220 oncology & carcinogenesis medicine Cancer research esophageal cancer long noncoding RNA Research Paper |
| Zdroj: | Journal of Cancer |
| ISSN: | 1837-9664 |
| DOI: | 10.7150/jca.26095 |
| Popis: | Esophageal cancer is one of the leading malignancies globally and long non-coding RNAs (lncRNAs) have been proved to have an important role in different malignancies including esophageal cancer. However their role in disease progression is still not clear. The objective of the study was to investigate the expression and role of LINC01234 in progression of esophageal cancer cells. LncRNA LINC01234 was found to be upregulated in esophageal cancer cells by chip sequencing. The expression level of LINC01234 was detected from different esophageal cancer cell lines by qRT-PCR. After this, the LINC01234 knockdown effects on cell proliferation, migration, invasion, and apoptosis were evaluated by cell proliferation assay, wound healing assay, invasion assay, and flow cytometric analysis in vitro. Expression of lncRNA LINC01234 was found to be markedly upregulated in the CEC2 cell line. Furthermore, cell proliferation, migration and invasion were significantly (P < 0.05) suppressed as compared to negative control while apoptotic rate was also found increased as a result of the knockdown of LINC01234. Significantly upregulated expression of LINC01234 in CEC2 cells and downregulated expression after knockdown is observed. The impact of LINC01234 knockdown on cell migration, invasion, proliferation and apoptosis indicated that LINC01234 may represent a new marker and a potential therapeutic target for esophageal cancer. |
| Databáze: | OpenAIRE |
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