Pseudomonas aeruginosa infection stimulates mitogen-activated protein kinases signaling pathway in human megakaryocytes
| Autor: | Gopi Krishnan Gopala Krishnan, Maheswaran Mani, Pavithra Vellaichamy, Aiswarya Sethumadhavan |
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| Rok vydání: | 2019 |
| Předmět: |
Lipopolysaccharides
Cell type p38 mitogen-activated protein kinases Immunology Down-Regulation Biology medicine.disease_cause Microbiology p38 Mitogen-Activated Protein Kinases Cell Line 03 medical and health sciences Immune system Downregulation and upregulation Virology medicine Humans Pseudomonas Infections 030304 developmental biology Mitogen-Activated Protein Kinase Kinases 0303 health sciences Innate immune system 030306 microbiology Pseudomonas aeruginosa Kinase Gene Expression Profiling JNK Mitogen-Activated Protein Kinases Cytotoxicity Tests Immunologic Immunity Innate Cell biology Signal transduction Mitogen-Activated Protein Kinases Megakaryocytes Signal Transduction |
| Zdroj: | Microbiology and immunology. 63(6) |
| ISSN: | 1348-0421 |
| Popis: | Pseudomonas aeruginosa is a major cause of nosocomial infections and contributes to higher mortality in hospitalized individuals. Infection by P. aeruginosa triggers host immune response through activation of pathogen recognition receptors, which are present in innate cells. Several studies have reported the mechanism of P. aeruginosa induced innate immunity in multiple cell types. But so far there is no reports on response of megakaryocytes to P. aeruginosa infection. Hence, our aim was to investigate the precise role and signaling mechanism of megakaryocytes during P. aeruginosa infection. In this study, we used Mo7e cells as representatives of human megakaryocyte and found that P. aeruginosa infection induces cytotoxicity in these cells. We further demonstrated that P. aeruginosa infection modulates p38 and extracellular signal regulated kinase pathways in Mo7e cells. Protein expression profiling in P. aeruginosa lipopolysaccharide-treated Mo7e cells revealed upregulation of importin subunit β and downregulation of metabolic enzymes. Our results suggest that P. aeruginosa infection regulates mitogen-activated protein kinases signaling pathway and importin in Mo7e cells and that this is a potential mechanism for nuclear translocation of nuclear factor binding near the κ light-chain gene in B cells and c-Jun N-terminal kinases to induce cell cytotoxicity. |
| Databáze: | OpenAIRE |
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