A multicentre analytical comparison study of inter-reader and inter-assay agreement of four programmed death-ligand 1 immunohistochemistry assays for scoring in triple-negative breast cancer
| Autor: | Wilko Weichert, Peter Sinn, Ulrich Sommer, Gustavo B. Baretton, Aurelia Noske, Mike Flores, Katja Steiger, Carsten Denkert, Hans-Heinrich Kreipe, Daniel-Christoph Wagner, Annette Lebeau, Marion Kiechle, Wilfried Roth, Stefanie Hieke-Schulz, Johannes Ammann |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Oncology Male medicine.medical_specialty Histology Concordance Triple Negative Breast Neoplasms B7-H1 Antigen Pathology and Forensic Medicine Cohort Studies 03 medical and health sciences 0302 clinical medicine Breast cancer Lymphocytes Tumor-Infiltrating Internal medicine medicine Biomarkers Tumor Humans Triple-negative breast cancer Aged Reproducibility Whole Genome Sequencing business.industry Cancer High-Throughput Nucleotide Sequencing Reproducibility of Results General Medicine Middle Aged medicine.disease Immunohistochemistry ddc 030104 developmental biology 030220 oncology & carcinogenesis Mutation Comparison study Female Neoplasm Grading business Programmed death |
| Zdroj: | HistopathologyReferences. 78(4) |
| ISSN: | 1365-2559 |
| Popis: | AIMS Studies in various cancer types have demonstrated discordance between results from different programmed death-ligand 1 (PD-L1) assays. Here, we compare the reproducibility and analytical concordance of four clinically developed assays for assessing PD-L1-positivity in tumour-infiltrating immune cells in the tumour area (PD-L1-IC-positivity) in triple-negative breast cancer (TNBC). METHODS AND RESULTS Primary TNBC resection specimens (n = 30) were selected based on their PD-L1-IC-positivity per VENTANA SP142 ( 5%: eight cases). Serial histological sections were stained for PD-L1 using VENTANA SP142, VENTANA SP263, DAKO 22C3 and DAKO 28-8. PD-L1-IC-positivity and tumour cell expression (≥1 versus |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text