3D high- and super-resolution imaging using single-objective SPIM
| Autor: | Vincent Studer, Jean-Baptiste Sibarita, Rémi Galland, Ajay Aravind, Gianluca Grenci, Virgile Viasnoff |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Materials science Photon business.industry Inverted microscope Cell Biology Laser Biochemistry Superresolution law.invention Single objective Optics Imaging Three-Dimensional Microscopy Fluorescence Multiphoton law Light sheet fluorescence microscopy Microscopy Animals Drosophila business Molecular Biology Biotechnology |
| Zdroj: | Nature methods. 12(7) |
| ISSN: | 1548-7105 |
| Popis: | Single-objective selective-plane illumination microscopy (soSPIM) is achieved with micromirrored cavities combined with a laser beam-steering unit installed on a standard inverted microscope. The illumination and detection are done through the same objective. soSPIM can be used with standard sample preparations and features high background rejection and efficient photon collection, allowing for 3D single-molecule-based super-resolution imaging of whole cells or cell aggregates. Using larger mirrors enabled us to broaden the capabilities of our system to image Drosophila embryos. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|