Enzymatic profiling of tetanus and botulinum neurotoxins based on vesicle-associated-membrane protein derived fluorogenic substrates
| Autor: | Daniel C. Pimenta, Maria A. Juliano, Sally M. A. Prado, Elen Aquino Perpetuo, Fernando Fratelli, Ivo Lebrun, L. Juliano |
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| Rok vydání: | 2008 |
| Předmět: |
Botulinum Toxins
Synaptobrevin Molecular Sequence Data Biology medicine.disease_cause Biochemistry R-SNARE Proteins Mice Tetanus Toxin Structural Biology medicine Fluorescence Resonance Energy Transfer Neurotoxin Syntaxin Animals Amino Acid Sequence Botulinum Toxins Type A Peptide sequence chemistry.chemical_classification Metalloproteinase Toxin Hydrolysis Metalloendopeptidases General Medicine Molecular biology Kinetics Enzyme Vesicle-associated membrane protein chemistry Peptides |
| Zdroj: | Protein and peptide letters. 15(10) |
| ISSN: | 0929-8665 |
| Popis: | Botulinum (BoNT) and tetanus (TeNT) neurotoxins are bacterial zinc metalloproteases that cleave and inactivate cellular proteins essential for neurotransmitter release. There are seven serotypes of BoNT, while TeNT is found in one serotype. In order to characterize their enzymatic activities and to propose serotype-differentiation an enzymatic assay based on their metalloprotease activity was developed. The assays were conducted with FRET peptides derived from SNAP-25, synaptobrevin and syntaxin. The substrates were cleaved by 2 ng/mL of toxin at different rates (K(cat)/K(M) from 0.028 to 75.9 microM.s(-)) at a single bond, as confirmed by Q-TOF mass spectrometry. Inhibition of the hydrolysis was obtained with EDTA or with specific antibodies directed to each neurotoxin. Different substrate selectivities, especially by BoNT- A and E, suggest that these substrates can be used as a putative method for clostridial toxin quantification and serotype differentiation and could be easily adapted to a high-throughput protocols. |
| Databáze: | OpenAIRE |
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