Sperm DNA integrity in testicular cancer patients
| Autor: | Eva Cavallin-Ståhl, K Jepson, Magdalena Cwikiel, Olof Ståhl, Aleksander Giwercman, Jakob Eberhard, Marcello Spanò |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male medicine.medical_specialty DNA damage Antineoplastic Agents Semen Biology Testicle Andrology Testicular Neoplasms Risk Factors In Situ Nick-End Labeling medicine Humans Longitudinal Studies Radiation Injuries Testicular cancer Gynecology TUNEL assay Radiotherapy Rehabilitation Obstetrics and Gynecology Cancer Neoplasms Germ Cell and Embryonal medicine.disease Spermatozoa Sperm Chromatin medicine.anatomical_structure Reproductive Medicine DNA fragmentation DNA Damage |
| Zdroj: | Human Reproduction. 21:3199-3205 |
| ISSN: | 1460-2350 0268-1161 |
| DOI: | 10.1093/humrep/del292 |
| Popis: | BACKGROUND: We evaluated the impact of testicular germ cell cancer (TGCC), its treatment and length of follow-up on sperm DNA integrity. METHODS: In 96 TGCC patients, semen was collected at specific intervals until 5 years after treatment. Sperm DNA integrity was assessed by the sperm chromatin structure assay (SCSA, n = 193) and by the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL, n = 159) assay. Results were expressed as DNA fragmentation index (DFI). Controls comprised of 278 military conscripts. RESULTS: Postsurgery testicular cancer (TC) patients did not differ from controls. Compared with pretreatment values, radiotherapy induced a transient increase in SCSA DFI (medians: 12 versus 19%; P = 0.03), normalizing after 3–5 years. One year or more after therapy, 5/13 (38%) of normozoospermic, irradiated patients had SCSADFI >27% compared with 7% of normozoospermic controls (P = 0.002). More than two cycles of chemotherapy decreased DFI 3–5 years posttherapy (median SCSADFI: 12 versus 9.1%, P = 0.02; median TUNELDFI: 11 versus 7.5%, P = 0.03). CONCLUSION: Irradiation increases sperm DNA damage 1–2 years after treatment, and 38% of irradiated patients with normozoospermia had high (>27%) DNA damage, which may affect the sperm-fertilizing ability. TC per se is not associated with an increase of DFI, and DFI is reduced by three or more cycles of chemotherapy. |
| Databáze: | OpenAIRE |
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