The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator of C. elegans development
| Autor: | Aleš Benda, Zdeněk Kostrouch, M Kostrouchová, Jan Philipp Novotný, Veronika Kostrouchová, Michael W. Krause, David Kostrouch, Marta Kostrouchová, Filip Kaššák, Vladimir Saudek, Ahmed Ali Chughtai |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
MED28 Evolution Protein subunit Mutant lcsh:Medicine Development General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Mediator Transcription (biology) Mediator complex Caenorhabditis elegans Molecular Biology Gene Gene expression regulation Regulation of gene expression Genetics biology General Neuroscience lcsh:R Cell Biology General Medicine biology.organism_classification Evolutionary Studies 030104 developmental biology General Agricultural and Biological Sciences Developmental Biology |
| Zdroj: | PeerJ, Vol 5, p e3390 (2017) PeerJ |
| DOI: | 10.7287/peerj.preprints.2530v1 |
| Popis: | The evolutionarily conserved Mediator complex is a critical player in regulating transcription. Comprised of approximately two dozen proteins, the Mediator integrates diverse regulatory signals through direct protein-protein interactions that, in turn, modulate the influence of Mediator on RNA Polymerase II activity. One Mediator subunit, MED28, is known to interact with cytoplasmic structural proteins, providing a potential direct link between cytoplasmic dynamics and the control of gene transcription. Although identified in many animals and plants, MED28 is not present in yeast; no bona fide MED28 has been described previously inCaenorhabditis elegans.Here, we identify bioinformatically F28F8.5, an uncharacterized predicted protein, as the nematode homologue of MED28. As in other Metazoa, F28F8.5 has dual nuclear and cytoplasmic localization and plays critical roles in the regulation of development.F28F8.5is a vital gene and its null mutants have severely malformed gonads and do not reproduce. F28F8.5 interacts on the protein level with the Mediator subunits MDT-6 and MDT-30. Our results indicate that F28F8.5 is an orthologue of MED28 and suggest that the potential to link cytoplasmic and nuclear events is conserved between MED28 vertebrate and nematode orthologues. |
| Databáze: | OpenAIRE |
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