Squamous cell lung carcinomas - the role of nm23-H1 gene
| Autor: | Jasminka Pavelić, Simun Krizanac, Krešimir Pavelić, Maja Herak Bosnar, Slobodnjak Z |
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| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Male
Pathology medicine.medical_specialty Lung Neoplasms Cell Loss of Heterozygosity Biology medicine.disease_cause nm23-H1 gene squamous cell lung carcinomas Loss of heterozygosity Drug Discovery medicine Humans Dinucleotide Repeats Genetics (clinical) Aged Monomeric GTP-Binding Proteins Lung Polymorphism Genetic Respiratory disease DNA Neoplasm Middle Aged NM23 Nucleoside Diphosphate Kinases medicine.disease Molecular medicine Immunohistochemistry medicine.anatomical_structure Epidermoid carcinoma Nucleoside-Diphosphate Kinase Carcinoma Squamous Cell Molecular Medicine Carcinogenesis Immunostaining Genes Neoplasm Transcription Factors |
| Popis: | This analysis of 32 pairs of human squamous cell lung carcinomas and normal matched control DNA demonstrates that loss of heterozygosity (LOH) is infrequent at the nm23-H1 locus, affecting only 2 of the 18 informative cases. Both LOH cases were in the tumor stage IIIA. One tumor was of poor and the other of moderate histological grade. These and an additional 34 tumor samples were also analyzed immunohistochemically for the presence of nm23-H1 protein. Of the 66 cases tested for the presence of nm23-H1 protein 54 were negative. Eight samples exhibited up to 35% positive cells (with weak immunostaining intensity) and four between 35% and 70% (moderate immunostaining intensity) ; no sample showed more than 70% positive cells. Noncancerous lung parts contained no nm23-H1 protein. nm23-H1 expression was independent of TNM stage, grade, tumor size, and patient"s survival. Two samples with LOH were negative for nm23-H1 protein. We therefore conclude that neither loss of heterozygosity of the nm23-H1 gene nor the intensity of specific protein expression are related to squamous cell lung carcinoma development and progression. [References: 18] |
| Databáze: | OpenAIRE |
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