Nuclear Morphometric Analysis (NMA) : screening of senescence, apoptosis and nuclear irregularities
| Autor: | Eduardo C. Filippi-Chiela, Vinicius Duval da Silva, Bruno Jurkovski, Guido Lenz, Sidia M. Callegari-Jacques, Manuel M. Oliveira |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Analise de dados [Estatistica]
lcsh:Medicine Apoptosis Molecular Cell Biology Basic Cancer Research Signaling in Cellular Processes lcsh:Science Mitotic catastrophe Apoptotic Signaling Cascade Cellular Senescence Apoptotic Signaling Etoposide Principal Component Analysis education.field_of_study Multidisciplinary Cell Death Chromosome Biology Caspase Inhibitors Cellular Structures Signaling Cascades Cell biology Phenotype medicine.anatomical_structure Oncology Vincristine Caspases Colonic Neoplasms Medicine Cell aging Research Article Signal Transduction Senescence Cell type Annexins Population Mitosis Biology Cell Line Tumor medicine Humans education Cell Nucleus lcsh:R Cell nucleus lcsh:Q Cisplatin HeLa Cells |
| Zdroj: | Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS PLoS ONE, Vol 7, Iss 8, p e42522 (2012) PLoS ONE |
| Popis: | Several cellular mechanisms affect nuclear morphology which can therefore be used to assess certain processes. Here, we present an analytic tool to quantify the number of cells in a population that present characteristics of senescence, apoptosis or nuclear irregularities through nuclear morphometric analysis. The tool presented here is based on nuclear image analysis and evaluation of size and regularity of adhered cells in culture. From 46 measurements of nuclear morphometry, principal component analysis filtered four measurements that best separated regular from irregular nuclei. These measurements, namely aspect, area box, radius ratio and roundness were combined into a single nuclear irregularity index (NII). Normal nuclei are used to set the parameters for a given cell type, and different nuclear phenotypes are separated in an area versus Nll plot. The tool was validated with B-gal staining for senescence and annexin or caspases inhibitor for apoptosis as well as severa I treatments that induce di fferent cellular phenotypes. This method provides a direct and objective way of screening normal, senescent, apoptotic and nuclear irregularities which may occur during failed mitosis or mitotic catastrophe, which may be very useful in basic and clinicai research. |
| Databáze: | OpenAIRE |
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