The localization of auxin transporters PIN3 and LAX3 during lateral root development in Arabidopsis thaliana
| Autor: | Francine Perrine-Walker, E. Jublanc |
|---|---|
| Přispěvatelé: | Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), La Trobe Institute of Molecular Sciences, La Trobe University [Melbourne], Dynamique Musculaire et Métabolisme (DMEM), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), La Trobe University, Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
Auxin influx Yellow fluorescent protein Auxin efflux Fluorophore [SDV]Life Sciences [q-bio] spectral imaging Plant Science Horticulture GFP 01 natural sciences linear unmixing Green fluorescent protein 03 medical and health sciences chemistry.chemical_compound Auxin Botany Arabidopsis thaliana [SDV.BV]Life Sciences [q-bio]/Vegetal Biology [INFO]Computer Science [cs] ComputingMilieux_MISCELLANEOUS 030304 developmental biology YFP chemistry.chemical_classification 0303 health sciences biology Lateral root fungi food and beverages biology.organism_classification Cell biology chemistry biology.protein 010606 plant biology & botany |
| Zdroj: | Biologia Plantarum Biologia Plantarum, Springer Verlag, 2014, 58 (4), pp.778-782. ⟨10.1007/s10535-014-0446-1⟩ |
| ISSN: | 0006-3134 1573-8264 |
| Popis: | International audience; Fluorophore tagged proteins are used in Arabidopsis thaliana to understand their functional role in plant development. This requires the analysis of their spatial localization in planta. However, the localization analysis is often perturbed by a significant overlap of the fluorophores used to label proteins of interest and the optical filtering methods available on the confocal microscope. This problem can be addressed by the use of spectral imaging with linear unmixing the image data. We applied this method to help us identify double transgenic A. thaliana lines which expressed two fluorescently tagged auxin transporter proteins: the auxin efflux protein PIN-FORMED-3 (PIN3), tagged with green fluorescent protein (GFP), and the auxin influx protein LIKE-AUX1-3 (LAX3), tagged with yellow fluorescent protein (YFP). This method allows the reliable separation of overlapping GFP and YFP fluorescence signals and subsequent localization analysis highlighting the potential benefit of this methodology in studies of lateral root development. |
| Databáze: | OpenAIRE |
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