A convenient method for preparation of the calcium ion-binding protein annexin V

Autor:	Hiroshi Mizokami, Chuichi Hirayama, Kenji Soejima, Tatsufumi Onchi, Keishin Sugawara, Shinichi Furukawa, Satoshi Koyanagi, Toshihiro Maeda, Hideo Yoshizaki
Rok vydání:	2004
Předmět:	Chromatography Binding protein Ion chromatography chemistry.chemical_element Bioengineering Calcium Applied Microbiology and Biotechnology Ion law.invention Adsorption chemistry Annexin law Recombinant DNA Calcium ion binding Biotechnology
Zdroj:	Journal of Bioscience and Bioengineering. 97:95-98
ISSN:	1389-1723
DOI:	10.1016/s1389-1723(04)70174-7
Popis:	This study presents a novel method for the production and purification of annexin V using cation exchange chromatography in the presence of calcium ions at neutral or alkaline pH. This method enables the handling of a large quantity of sample at one time without deterioration of the adsorption capacity of the cation exchange carrier by contaminating proteins, as well as the production of the calcium ion-binding protein annexin V with high purity on an industrial scale from both recombinant products and native products.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe6120dbfe036c61f36b85bf1bf20f70 https://doi.org/10.1016/s1389-1723(04)70174-7 Zobrazit plný text záznamu Full Text from ScienceDirect