Applicability of random cloning method to analyze microbial community in full-scale anaerobic digesters
| Autor: | Shuichi Mori, Jihoon Cheon, Hiromoto Koshikawa, Hiroshi Tsuno, Taira Hidaka |
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| Rok vydání: | 2008 |
| Předmět: |
Microbial metabolism
Bioengineering substrate Biology Applied Microbiology and Biotechnology DNA Ribosomal Polymerase Chain Reaction Bioreactors 16S rDNA RNA Ribosomal 16S Bioreactor Anaerobiosis Cloning Molecular DNA Primers Bacteria Base Sequence Ecology Thermophile full-scale temperature 16S ribosomal RNA Pulp and paper industry random cloning Substrate (marine biology) Microbial population biology anaerobic digester lab-scale microbial community Sludge Biotechnology Mesophile |
| Zdroj: | Journal of bioscience and bioengineering. 106(2) |
| ISSN: | 1347-4421 |
| Popis: | Microbial communities were analyzed in six full-scale anaerobic digesters and a lab-scale digester using the random cloning method. The purposes were to confirm the applicability, reproducibility, and error range of this method; to discuss the difference in the dominant microbes determined by this method in different operational conditions of temperature (mesophilic and thermophilic) and substrate (garbage, sewage sludge, and livestock waste); and to determine key microbes in each digester. Each sample was analyzed in triplicate. In one of the samples, 373 clones were analyzed to study the composition of microbial community in the digester. Time course analysis was conducted from the start-up period for approximately one year in one of the digesters. Similar microbial diversity was obtained corresponding to the type of substrate change (sewage sludge to garbage). Operational taxonomic units (OTUs) closely related to Coprothermobacter sp. and unidentified bacterium clones TUG14 and TUG22 disappeared during the first 40 d, while OTUs closely related to Bacillus sp. and Clostridium sp. increased later. Microbial diversity in digesters is strongly affected by the operational conditions, and similar microbial diversity can be obtained in triplicate analysis and under similar operational conditions. The present study verified the applicability of this method to discuss the overall difference in microbial communities. |
| Databáze: | OpenAIRE |
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