Eukaryotic expression: developments for structural proteomics

Autor: Albrecht Messerschmidt, Udo Heinemann, Simon J. Davis, S. Macieira, Tzviya Zeev-Ben-Mordehai, D Ksiazek, Yoav Peleg, Titia K. Sixma, Geoff Sutton, Klaus Maskos, Ian M. Jones, E Y Jones, G Schneider, Rene Assenberg, N Tarboureich, Arnaud Poterszman, Didier Busso, Joel L. Sussman, Lena Gustafsson, A R Aricescu, Christine Lang, Anastassis Perrakis, Veronica T. Chang, A Dubrovsky, Roslyn M. Bill, Kristina Hedfalk
Přispěvatelé: Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Proteomics
Glycosylation
MESH: Gene Expression
Saccharomyces cerevisiae
Gene Expression
MESH: Selenomethionine
Computational biology
high throughput
yeast
MESH: Eukaryotic Cells
mammalian cells
Pichia pastoris
03 medical and health sciences
chemistry.chemical_compound
baculovirus
Structural Biology
Yeasts
Gene expression
Protein biosynthesis
Animals
MESH: Animals
MESH: Cloning
Molecular
Cloning
Molecular
Selenomethionine
Cells
Cultured
030304 developmental biology
Cloning
0303 health sciences
biology
MESH: Proteomics
MESH: Yeasts
030302 biochemistry & molecular biology
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
General Medicine
biology.organism_classification
MESH: Glycosylation
Research Papers
eukaryotic expression
Yeast
Cell biology
Eukaryotic Cells
chemistry
MESH: Baculoviridae
Baculoviridae
MESH: Cells
Cultured
Zdroj: Acta Crystallographica Section D: Biological Crystallography
Acta Crystallographica Section D: Biological Crystallography, International Union of Crystallography, 2006, 62 (Pt 10), pp.1114-24. ⟨10.1107/S0907444906029805⟩
Acta Crystallographica Section D: Biological Crystallography, 62, 1114. International Union of Crystallography
ISSN: 0907-4449
DOI: 10.1107/S0907444906029805⟩
Popis: The production of sufficient quantities of protein is an essential prelude to a structure determination, but for many viral and human proteins this cannot be achieved using prokaryotic expression systems. Groups in the Structural Proteomics In Europe (SPINE) consortium have developed and implemented high-throughput (HTP) methodologies for cloning, expression screening and protein production in eukaryotic systems. Studies focused on three systems: yeast (Pichia pastoris and Saccharomyces cerevisiae), baculovirus-infected insect cells and transient expression in mammalian cells. Suitable vectors for HTP cloning are described and results from their use in expression screening and protein-production pipelines are reported. Strategies for co-expression, selenomethionine labelling (in all three eukaryotic systems) and control of glycosylation (for secreted proteins in mammalian cells) are assessed.
Databáze: OpenAIRE
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