Spatiotemporal analysis of tumour-infiltrating immune cells in biliary carcinogenesis

Autor: Alphonse Charbel, Luca Tavernar, Thomas Albrecht, Fritz Brinkmann, Joanne Verheij, Eva Roos, Monika Nadja Vogel, Bruno Köhler, Christoph Springfeld, Alexander Brobeil, Peter Schirmacher, Stephan Singer, Arianeb Mehrabi, Stephanie Roessler, Benjamin Goeppert
Přispěvatelé: Pathology, CCA - Cancer biology and immunology
Rok vydání: 2023
Předmět:
Zdroj: Charbel, A, Tavernar, L, Albrecht, T, Brinkmann, F, Verheij, J, Roos, E, Vogel, M N, Köhler, B, Springfeld, C, Brobeil, A, Schirmacher, P, Singer, S, Mehrabi, A, Roessler, S & Goeppert, B 2022, ' Spatiotemporal analysis of tumour-infiltrating immune cells in biliary carcinogenesis ', British Journal of Cancer, vol. 127, no. 9, pp. 1603-1614 . https://doi.org/10.1038/s41416-022-01933-0
British journal of cancer, 127(9), 1603-1614. Nature Publishing Group
British Journal of Cancer, 127(9), 1603-1614. Nature Publishing Group
ISSN: 1439-7803
0007-0920
Popis: BackgroundIntraductal papillary neoplasms (IPN) and biliary epithelial neoplasia (BilIN) are well‐defined precursor lesions of biliary tract carcinoma (BTC). The aim of this study was to provide a comprehensive characterisation of the inflammatory microenvironment in BTC precursor lesions.MethodsImmunohistochemistry was employed to assess tumour-infiltrating immune cells in tissue samples from patients, for whom precursor lesions were identified alongside invasive BTC. The spatiotemporal evolution of the immune microenvironment during IPN-associated carcinogenesis was comprehensively analysed using triplet sample sets of non-neoplastic epithelium, precursor lesion and invasive BTC. Immune-cell dynamics during IPN- and BilIN-associated carcinogenesis were subsequently compared.ResultsStromal CD3+(P = 0.002), CD4+(P = 0.007) and CD8+(P +B cells (P = 0.008), MUM1+plasma cells (P = 0.012) and CD163+M2-like macrophages (P = 0.008) significantly decreased in IPN compared to non-tumorous biliary epithelium. Upon transition from IPN to invasive BTC, stromal CD68+(P = 0.001) and CD163+(P +T-lymphocytic infiltration from non-tumorous epithelium via BilIN (P = 0.008) to BTC (P = 0.004).ConclusionIPN and BilIN are immunologically distinct entities that undergo different immune-cell variations during biliary carcinogenesis. Intraepithelial CD8+T-lymphocytic infiltration of biliary tissue decreased already at the IPN-precursor stage, whereas BilIN-associated carcinogenesis showed a slowly progressing reduction towards invasive carcinoma.
Databáze: OpenAIRE
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