Effect of Vascular Endothelial Growth Factor on Nitric Oxide Production by Cultured Rat Mesangial Cells
| Autor: | Howard Trachtman, Nicholas Franki, Stephen Futterweit, Pravin C. Singhal |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty Cell Survival Biophysics Nitric Oxide Synthase Type II Endothelial Growth Factors Nitric Oxide Biochemistry Nitric oxide chemistry.chemical_compound Diabetic Neuropathies In vivo Proto-Oncogene Proteins Internal medicine medicine Animals Receptors Growth Factor RNA Messenger Enzyme Inhibitors Molecular Biology Cells Cultured Lymphokines Vascular Endothelial Growth Factor Receptor-1 Mesangial cell biology Vascular Endothelial Growth Factors Lymphokine Receptor Protein-Tyrosine Kinases Cell Biology Glomerular Mesangium Rats Nitric oxide synthase Vascular endothelial growth factor Vascular endothelial growth factor A Glucose NG-Nitroarginine Methyl Ester Receptors Vascular Endothelial Growth Factor Endocrinology chemistry biology.protein Nitric Oxide Synthase Tyrosine kinase |
| Zdroj: | Biochemical and Biophysical Research Communications. 245:443-446 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1998.8454 |
| Popis: | Vascular endothelial growth factor (VEGF) stimulates nitric oxide (NO) production by endothelial cells in vitro and in vivo. However, the impact of VEGF on inducible nitric oxide synthase (iNOS) activity and NO synthesis in cultured mesangial cells is not known. Therefore, we measured nitrite accumulation in cytokine-stimulated, rat mesangial cells (RMC) in response to graded concentrations of VEGF. Addition of VEGF (10-50 ng/ml) did not alter RMC viability or NO production in either normal (5.6 mM) or high (33.3 mM) glucose conditions. Exposure of RMC to VEGF did not modify the effects of L-arginine (20 mM) or L-NAME (1 mM) on nitrite accumulation in normal or high glucose media. The steady state abundance of iNOS mRNA and the cytosolic content of iNOS protein were unaffected by addition of VEGF. Cultured RMC expressed the high-affinity tyrosine kinase VEGF receptors, flt and flk/KDR, and the levels were not modulated by incubation in normal or high glucose media. We conclude that VEGF does not regulate proliferation or NO production in cultured RMC. These findings suggest that disturbances in the normal interaction between VEGF and NO are not involved in the pathogenesis of abnormal mesangial cell structure or function in diabetic nephropathy. |
| Databáze: | OpenAIRE |
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