Ubiquitin carboxyl-terminal hydrolases are required for period maintenance of the circadian clock at high temperature in Arabidopsis
| Autor: | Federico Valverde, Richard D. Vierstra, Peizhen Yang, Ryosuke Hayama, Tsuyoshi Mizoguchi, George Coupland, Ikuyo Furutani-Hayama |
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| Přispěvatelé: | Valverde, Federico [0000-0001-8063-0974], Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular, Valverde, Federico |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hot Temperature Circadian clock Mutant Arabidopsis lcsh:Medicine 01 natural sciences Article 03 medical and health sciences Ubiquitin Gene Expression Regulation Plant Circadian Clocks Gene expression lcsh:Science Regulation of gene expression Multidisciplinary biology Deubiquitinating Enzymes Chemistry lcsh:R Ubiquitination Proteases biology.organism_classification Cell biology Gene regulation 030104 developmental biology Proteasome biology.protein lcsh:Q Signal transduction Ubiquitin Thiolesterase 010606 plant biology & botany Signal Transduction |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) Digital.CSIC. Repositorio Institucional del CSIC instname idUS. Depósito de Investigación de la Universidad de Sevilla Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Protein ubiquitylation participates in a number of essential cellular processes including signal transduction and transcription, often by initiating the degradation of specific substrates through the 26S proteasome. Within the ubiquitin-proteasome system, deubiquitylating enzymes (DUBs) not only help generate and maintain the supply of free ubiquitin monomers, they also directly control functions and activities of specific target proteins by modulating the pool of ubiquitylated species. Ubiquitin carboxyl-terminal hydrolases (UCHs) belong to an enzymatic subclass of DUBs, and are represented by three members in Arabidopsis, UCH1, UCH2 and UCH3. UCH1 and UCH2 influence auxin-dependent developmental pathways in Arabidopsis through their deubiquitylation activities, whereas biological and enzymatic functions of UCH3 remain unclear. Here, we demonstrate that Arabidopsis UCH3 acts to maintain the period of the circadian clock at high temperatures redundantly with UCH1 and UCH2. Whereas single uch1, uch2 and uch3 mutants have weak circadian phenotypes, the triple uch mutant displays a drastic lengthening of period at high temperatures that is more extreme than the uch1 uch2 double mutant. UCH3 also possesses a broad deubiquitylation activity against a range of substrates that link ubiquitin via peptide and isopeptide linkages. While the protein target(s) of UCH1-3 are not yet known, we propose that these DUBs act on one or more factors that control period length of the circadian clock through removal of their bound ubiquitin moieties, thus ensuring that the clock oscillates with a proper period even at elevated temperatures |
| Databáze: | OpenAIRE |
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