In HIV-1-infected patients, plasma levels of HIV-1 RNA are inversely correlated with IFN-α responsiveness of whole-blood cultures to sendai virus
| Autor: | L. Bocket, S Chabou, Gunnar V. Alm, Wassim Chehadeh, C Fontier, Georges Lion, Didier Hober, Y Mouton, Pierre Wattré |
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| Rok vydání: | 2000 |
| Předmět: |
HIV Infections
Virus Replication Respirovirus Virus Cell Line Immune system Interferon Virology medicine Animals Humans Whole blood biology Interferon-alpha RNA biology.organism_classification Molecular biology Sendai virus In vitro Culture Media Infectious Diseases Vesicular stomatitis virus HIV-1 RNA Viral Cattle medicine.drug |
| Zdroj: | Journal of Clinical Virology. 16:123-128 |
| ISSN: | 1386-6532 |
| DOI: | 10.1016/s1386-6532(99)00088-8 |
| Popis: | Background: A diminished or totally blocked IFN-α production in cells from HIV-1-infected patients has been reported. Objective: To investigate the relationship between the decreased in vitro production of IFN-α and the plasma level of HIV-1 RNA. Study design: Whole blood samples of 39 healthy subjects and 44 HIV-1-infected patients were incubated in the presence of Sendai virus for 24 h. IFN-α contained in supernatants was assayed by using an immunochemical method (DELFIA) and by using an antiviral assay. Plasma HIV-1 RNA was measured by the Amplicor HIV-1 monitor test. Results: The levels of IFN-α obtained were significantly lower in cultures from HIV-1 infected patients than in control subjects (P |
| Databáze: | OpenAIRE |
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