Hyperthermia enhances cytotoxicity of amine oxidase and spermine on drug-resistant LoVo colon adenocarcinoma cells
| Autor: | Laura Dalla Vedova, Francesca Belli, Giuseppe Arancia, Manuela Marra, Enzo Agostinelli, Pasqualina Crateri |
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| Rok vydání: | 2006 |
| Předmět: |
Cancer Research
Cell Survival Spermine Aldehyde dehydrogenase Adenocarcinoma Biology chemistry.chemical_compound Cell Line Tumor Humans Cytotoxic T cell Hyperthermia Cytotoxicity Monoamine Oxidase Hyperthermia Induced Kinetics Oncology Biochemistry chemistry Drug Resistance Neoplasm Apoptosis Cell culture Colonic Neoplasms Cancer cell Cancer research biology.protein Polyamine |
| Zdroj: | International Journal of Oncology. |
| ISSN: | 1791-2423 1019-6439 |
| DOI: | 10.3892/ijo.28.6.1543 |
| Popis: | Hyperthermia is currently receiving widespread attention when associated with other therapeutic modalities, such as irradiation or chemotherapy, in the treatment of cancer. The occurrence of resistance to cytotoxic pharmacological agents in tumor cells, associated with several phenotypic alterations, is one of the major obstacles to successful anticancer chemotherapy. We investigated a new strategy to overcome multidrug resistance (MDR) cancer cells, using bovine serum amine oxidase (BSAO), which forms toxic products from spermine (H2O2 and aldehydes). The cytotoxicity of the products was evaluated in drug-sensitive (LoVo WT) and multidrug-resistant (LoVo DX) colon adenocarcinoma cells at 37 and 42 degrees C, using a clonogenic cell survival assay. Cytotoxicity was considerably enhanced at 42 degrees C. Both toxic species contributed to the thermal enhancement of cytotoxicity induced by BSAO and spermine. Cytotoxicity was eliminated in the presence of catalase and aldehyde dehydrogenase (ALDH). An interesting finding was that BSAO and spermine at |
| Databáze: | OpenAIRE |
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