Cytokine-induced upregulation of NF-κB, IL-8, and ICAM-1 is dependent on colonic cell polarity: implication for PKCδ
| Autor: | Marie Pierre Montero, Claude Penel, Francis Fouchier, Sébastien Vallée, Serge Champion, Sullivan Laforest |
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| Přispěvatelé: | Cytosquelette et Intégration des Signaux du Micro-Environnement Tumoral - UMR 6032 (CISMET), Centre National de la Recherche Scientifique (CNRS)-Université de Provence - Aix-Marseille 1-Université de la Méditerranée - Aix-Marseille 2, Laboratoire de Physiologie Végétale, University of Geneva [Switzerland], Nutrition humaine et lipides : Biodisponibilité, métabolisme et régulation, Université de la Méditerranée - Aix-Marseille 2-Institut National de la Recherche Agronomique (INRA)-Université de Provence - Aix-Marseille 1-IFR125-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de la Méditerranée - Aix-Marseille 2-Université de Provence - Aix-Marseille 1-Centre National de la Recherche Scientifique (CNRS), Université de Genève = University of Geneva (UNIGE) |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Transcriptional Activation
medicine.medical_treatment [SDV]Life Sciences [q-bio] Chromosomal translocation Biology Adenocarcinoma Gene Expression Regulation Enzymologic 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Line Tumor Cell polarity medicine Humans Interleukin 8 Intestinal Mucosa Protein Kinase C ComputingMilieux_MISCELLANEOUS 030304 developmental biology Regulation of gene expression 0303 health sciences Microvilli Tumor Necrosis Factor-alpha Cell Membrane Interleukin-8 NF-kappa B Cell Polarity Cell Differentiation Epithelial Cells Cell Biology Drug Tolerance Intercellular Adhesion Molecule-1 Cell biology Up-Regulation Protein Kinase C-delta Protein Transport Cytokine Cell culture 030220 oncology & carcinogenesis Colonic Neoplasms Cytokines Tumor necrosis factor alpha Interleukin-1 |
| Zdroj: | Experimental Cell Research Experimental Cell Research, Elsevier, 2004, 297 (1), pp.165-185. ⟨10.1016/j.yexcr.2004.03.007⟩ Experimental Cell Research, 2004, 297 (1), pp.165-185. ⟨10.1016/j.yexcr.2004.03.007⟩ |
| ISSN: | 0014-4827 1090-2422 |
| DOI: | 10.1016/j.yexcr.2004.03.007⟩ |
| Popis: | As described for a long time, carcinoma-derived Caco-2 cells form a polarized epithelium in culture, whereas HT29-D4 cells are nonpolarized and undifferentiated but can form a polarized monolayer when cultured in a galactose-supplemented medium. Using NF-kappaB translocation and IL-8 and ICAM-1 gene activation as an index, we have studied the relationship between the differentiation state and the cell response to cytokines. We found that differentiated Caco-2 and HT29-D4 cells were responsive to both cytokines TNFalpha- and IL-1beta-mediated activation of NF-kappaB but that undifferentiated HT29-D4 cells were unresponsive to IL-1beta. However, the expression of endogenous ICAM-1 and IL-8 genes was upregulated by these cytokines in either cell lines differentiated or not. Upregulation of ICAM-1 gene occurred when IL-1beta or TNFalpha was added to the basal, but not apical surface of the differentiated epithelia. Finally, it appeared that in polarized HT29-D4 cells, the IL-1beta-induced translocation of NF-kappaB was connected to PKCdelta translocation. |
| Databáze: | OpenAIRE |
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