| Zdroj: |
Hestbjerg, H, Elmholt, S, Thrane, U & Jensen, U B 1999, ' A resource-saving method for isolation of Fusarium and other fungi from individual soil particles ', Mycological Research, vol. 103, pp. 1545-1548 . |
| Popis: |
Planting of individual soil particles onto an agar medium, poured into Petri dishes, is a method of studying fungal distribution between microhabitats in soil. The substitution of Petri dishes with well plates reduced both the required amount of agar medium and labour. A microshovel prepared from an injection needle facilitated the handling of individual, small size, soil particles (0.25–0.50 mm). The described method was evaluated in a study of the distribution of Fusarium within two wheat field soils from As, Norway, and from Ostermarie, Denmark. Four types of organic particles were distinguished: light coloured root pieces, dark coloured root pieces, straw pieces, and miscellaneous organic pieces. The dominating species were F. culmorum, F. oxysporum and F. avenaceum . Aspects on the value of the method with regard to fungal substrate preference are discussed. |
