Developmentally programmed expression of AID in chicken B cells
Autor: | David R. Withers, John R. Young, T. Fred Davison |
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Rok vydání: | 2005 |
Předmět: |
Immunoglobulin gene
animal structures Molecular Sequence Data Immunology Gene Conversion Somatic hypermutation Bursa of Fabricius Cytidine Deaminase Activation-induced (cytidine) deaminase Animals RNA Messenger Gene conversion B-Lymphocytes Base Sequence biology Germinal center Cell Differentiation Molecular biology Gene Expression Regulation Immunoglobulin class switching biology.protein Immunoglobulin Light Chains Antibody Immunoglobulin Gene Rearrangement Chickens Spleen Developmental Biology |
Zdroj: | Developmental & Comparative Immunology. 29:651-662 |
ISSN: | 0145-305X |
Popis: | In mice, activation induced deaminase, AID, is expressed only in germinal center B cells. It is required for the initiation of somatic hypermutation and class switch recombination. In chickens and most mammals immunoglobulin gene rearrangement generates limited diversity and the primary immunoglobulin repertoire depends on subsequent somatic hypermutation or gene conversion. Immunoglobulin gene conversion in chickens starts in the embryonic bursa, before antigen exposure. The demonstrated requirement for AID for gene conversion in the bursal lymphoma cell line, DT40, implies developmental regulation of AID expression. To test this prediction, we examined the timing and location of AID mRNA expression. An abrupt increase in AID mRNA coincided with the onset of extensive Ig gene conversion in the bursa. Expression was also detected at earlier stages, implying either that expression of AID is not the only controlling factor for gene conversion, or that gene conversion can precede the formation of bursal follicles. |
Databáze: | OpenAIRE |
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