Establishment of a localized acute implant-associated Staphylococcus aureus bone infection model in sheep
| Autor: | Salim E Darwiche, Agnieszka Karol, Yvonne Achermann, Katharina Siwy, Tiziano A. Schweizer, Karina Klein, Beat Lechmann, Brigitte von Rechenberg |
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| Přispěvatelé: | University of Zurich, Darwiche, Salim E |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Staphylococcus aureus medicine.medical_specialty Pathology Prosthesis-Related Infections 030106 microbiology 610 Medicine & health medicine.disease_cause 2726 Microbiology (medical) 10234 Clinic for Infectious Diseases Bone Infection Sepsis 03 medical and health sciences 0302 clinical medicine 2400 General Immunology and Microbiology Animals Humans Immunology and Allergy Medicine 030222 orthopedics Sheep General Immunology and Microbiology business.industry Osteomyelitis Soft tissue 2725 Infectious Diseases Prostheses and Implants General Medicine Staphylococcal Infections medicine.disease Disease Models Animal Infectious Diseases Lameness Biofilms Orthopedic surgery 2723 Immunology and Allergy Implant business |
| Zdroj: | Pathogens and Disease. 79 |
| ISSN: | 2049-632X |
| DOI: | 10.1093/femspd/ftab032 |
| Popis: | Orthopedic implant-associated bacterial infections with Staphylococcus aureus constitute a major clinical problem, and large pre-clinical animal models remain scarce. The aim of this study was to establish a standardized method of a localized, acute S. aureus bone infection in the presence of complex implanted devices in a sheep model. Four sheep underwent surgery receiving a complex implanted metallic device with a component stabilizing a bone defect created in the left tibial metaphysis, and an attached component placed in adjacent soft tissue. The bone defect was inoculated with S. aureus strain ATCC25293 (1 × 104 CFU). Twenty one days later, the surgery site was macroscopically evaluated, tissue samples and implants harvested for bacterial cell count quantification and tissue samples histologically analyzed. The animals exhibited clinical signs of localized infection (e.g. swelling, lameness, pain) but did not develop symptoms of sepsis. After euthanasia, macroscopic assessment revealed a localized bone and soft tissue infection at the surgery site. Histologically, an acute inflammation with neutrophils but also signs of bone destruction with necrosis was noted. An ovine model of a localized, acute S. aureus bone infection with complex implants was successfully established and could be used to test novel treatments against orthopedic implant-associated infections. |
| Databáze: | OpenAIRE |
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