Bacteria diversity and arsenic mobilization in rock biofilm from an ancient gold and arsenic mine
Autor: | Karolina Tomczyk-Żak, Urszula Zielenkiewicz, Aleksandra Sklodowska, Łukasz Dmoch, Łukasz Drewniak, Szymon Kaczanowski |
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Rok vydání: | 2013 |
Předmět: |
Biogeochemical cycle
Siderophore Environmental Engineering Molecular Sequence Data Siderophores chemistry.chemical_element Mining Arsenic RNA Ribosomal 16S Cluster Analysis Environmental Chemistry Microbial mat Cloning Molecular Waste Management and Disposal Phylogeny Alphaproteobacteria Likelihood Functions Base Sequence Models Genetic biology Reverse Transcriptase Polymerase Chain Reaction Ecology Biofilm Computational Biology Biodiversity Sequence Analysis DNA 16S ribosomal RNA biology.organism_classification Pollution Rhizobiales chemistry Genes Bacterial Biofilms Microscopy Electron Scanning Gold Poland Bacteria |
Zdroj: | Science of The Total Environment. :330-340 |
ISSN: | 0048-9697 |
DOI: | 10.1016/j.scitotenv.2013.04.087 |
Popis: | In this paper we characterize the biofilm community from an ancient Zloty Stok gold and arsenic mine. Bacterial diversity was examined using a culture-independent technique based on 16S rRNA gene amplification, cloning and sequencing. We show that unexpectedly the microbial diversity of this community was extremely high (more than 190 OTUs detected), with the most numerous members from Rhizobiales (α-Proteobacteria). Although the level of rock biofilm diversity was similar to the microbial mat community we have previously characterized in the same adit, its taxonomic composition was completely different. Detailed analysis of functional arrA and aioA genes, chemical properties of siderophores found in pore water as well as the biofilm chemical composition suggest that the biofilm community contributes to arsenic pollution of surrounding water in a biogeochemical cycle similar to the one observed in bacterial mats. To interpret our results concerning the biological arsenic cycle, we applied the theory of ecological pyramids of Charles Elton. |
Databáze: | OpenAIRE |
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