Analysis of Sequence Variation in Grapevine Yellow Speckle Viroid 1 Reveals Two Distinct Alternative Structures for the Pathogenic Domain
| Autor: | Justin E. Rigden, M.A. Rezaian |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Genetics
music.instrument Base Sequence biology Viroid viruses Molecular Sequence Data Nucleic acid sequence Genetic Variation RNA biology.organism_classification Virology Viroids Plant Viruses Fruit Complementary DNA Grapevine yellow speckle viroid Nucleic Acid Conformation RNA Viral Genetic variability Cloning Molecular music Protein secondary structure Sequence (medicine) |
| Zdroj: | Virology. 193:474-477 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/s0042-6822(15)80001-4 |
| Popis: | The nucleotide sequences of 24 full-length eDNA clones prepared from a field isolate of grapevine yellow speckle viroid 1 (GYSV 1) have been determined and compared with that of the previously published GYSV 1 sequence. A large number of sequence variations were observed, the majority of which occurred in the pathogenicity domain (P domain) of the viroid. The GYSV 1 variants could be divided into two types each containing a distinct secondary structure within the P domain. Monomeric exact-length RNA transcripts produced from individual clones belonging to both types were infectious in viroid-free grapevines and produced homogenous populations of progeny viroid in vivo . |
| Databáze: | OpenAIRE |
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