Balance between pro and anti-inflammatory cytokines in patients with acute alcoholic hepatitis
| Autor: | Axel Balian, Frédérique Capron, Liliane Grangeot-Keros, Hélène Agostini, David Fallik, Pierre Galanaud, Dominique Emilie, Alain Portier, Jean-Claude Chaput, Bruno Raynard, Sylvie Naveau |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Alcoholic liver disease Cirrhosis Biopsy Prednisolone Receptors Tumor Necrosis Factor Pathogenesis Liver disease Proliferating Cell Nuclear Antigen medicine Humans Prospective Studies Glucocorticoids Liver Diseases Alcoholic Liver injury Hepatitis business.industry Gastroenterology General Medicine Middle Aged medicine.disease Interleukin-10 Up-Regulation Fatty Liver Liver Case-Control Studies Immunology Acute Disease Female Steatosis Acute Alcoholic Hepatitis business |
| Zdroj: | Gastroenterologie clinique et biologique. 29(3) |
| ISSN: | 0399-8320 |
| Popis: | The ability of endogenous IL-10 to modulate inflammatory response and to limit hepatotoxicity has been shown in several models of liver injury.The objectives of this study were to evaluate the relationship between liver disease and the balance between pro and anti-inflammatory cytokines in acute alcoholic hepatitis.Twenty-five patients with pure steatosis, 17 with cirrhosis and mild acute alcoholic hepatitis (discriminant function value32) and 41 patients with cirrhosis and severe acute alcoholic hepatitis (discriminant function value=32) were studied. Plasma levels of interleukin 10 (IL-10) and soluble TNF receptors (TNFsRp75 and 55) were analyzed using ELISA assays. Hepatocyte proliferative activity was assessed with proliferating cell nuclear antigen labeling index (PCNA-LI) on formalin-fixed paraffin embedded liver biopsy specimens.In patients with steatosis, cirrhosis with mild and severe acute alcoholic hepatitis, the plasma levels of IL-10 were higher (P0.05) than in healthy controls. Between day 1 and day 8, the TNFsRp55/IL-10 ratio increased by 137 +/- 47 in the 10 patients with severe acute alcoholic hepatitis treated with prednisolone who died within 2 months and by 9.3 +/- 14 in the 19 patients still alive at 2 months (P=0.031). In patients with severe acute alcoholic hepatitis, PCNA-LI on liver biopsy was negatively correlated with the TNFsRp55/IL-10 ratio increase from day 1 to day 8 (r=- 0.42, P=0.11). PCNA-LI was positively correlated with TNFsRp75/TNFsRp 55 ratio increase from day 1 to day 15 (r=0.52; p0.05).Our data suggest the anti-inflammatory system is up-regulated in patients with alcoholic liver disease. Nevertheless, in patients with severe acute alcoholic hepatitis, IL-10 production seems insufficient to modulate TNF-alpha cytotoxicity mediated by TNFRp55. |
| Databáze: | OpenAIRE |
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