Post microtextures accelerate cell proliferation and osteogenesis
| Autor: | Shuvo Roy, George F. Muschler, Alvaro Mata, Aaron J. Fleischman, Cynthia Boehm, Eun Jung Kim |
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| Rok vydání: | 2010 |
| Předmět: |
Time Factors
Surface Properties Cellular differentiation Blotting Western Cell Culture Techniques Biomedical Engineering Connective tissue Bone Marrow Cells Biology Biochemistry Article Extracellular matrix Biomaterials Osteogenesis medicine Humans Dimethylpolysiloxanes Progenitor cell Molecular Biology Connective tissue progenitor cells Cell Proliferation Adult stem cells Polydimethylsiloxane Soft lithography Reverse Transcriptase Polymerase Chain Reaction Stem Cells Cell Differentiation General Medicine Alkaline Phosphatase Molecular biology Cell biology Extracellular Matrix Nylons medicine.anatomical_structure Cell culture Connective Tissue Alkaline phosphatase Microfabrication Stem cell Adult stem cell Biotechnology |
| Zdroj: | Acta Biomaterialia. 6(1):160-169 |
| ISSN: | 1742-7061 |
| DOI: | 10.1016/j.actbio.2009.06.016 |
| Popis: | The influence of surface microtexture on osteogenesis was investigated in vitro by examining the proliferation and differentiation characteristics of a class of adult stem cells and their progeny, collectively known as connective tissue progenitor cells (CTPs). Human bone marrow-derived CTPs were cultured for up to 60 days on smooth polydimethylsiloxane (PDMS) surfaces and on PDMS with post microtextures that were 10 microm in diameter and 6 microm in height, with 10 microm separation. DNA quantification revealed that the numbers of CTPs initially attached to both substrates were similar. However, cells on microtextured PDMS transitioned from lag phase after 4 days of culture, in contrast to 6 days for cells on smooth surfaces. By day 9 cells on the smooth surfaces exhibited arbitrary flattened shapes and migrated without any preferred orientation. In contrast, cells on the microtextured PDMS grew along the array of posts in an orthogonal manner. By days 30 and 60 cells grew and covered all surfaces with extracellular matrix. Western blot analysis revealed that the expression of integrin alpha5 was greater on the microtextured PDMS compared with smooth surfaces. Real time reverse transcription-polymerase chain reaction revealed that gene expression of alkaline phosphatase had decreased by days 30 and 60, compared with that on day 9, for both substrates. Gene expression of collagen I and osteocalcin was consistently greater on post microtextures relative to smooth surfaces at all time points. |
| Databáze: | OpenAIRE |
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