Expression Analysis of Angiogenesis-Related Genes in Bulgarian Patients with Early-Stage Non-Small Cell Lung Cancer

Autor: Svetlana Nikolova Metodieva, Radostina Cherneva, Draga Toncheva, Danail Petrov, Dragomira Nikolova, Ivanka Dimova
Rok vydání: 2011
Předmět:
Adult
Male
Cancer Research
Lung Neoplasms
Angiogenesis
Pilot Projects
Adenocarcinoma
Biology
Polymerase Chain Reaction
030218 nuclear medicine & medical imaging
Neovascularization
03 medical and health sciences
0302 clinical medicine
Carcinoma
Non-Small-Cell Lung
Biomarkers
Tumor
medicine
Carcinoma
Humans
Bulgaria
Lung cancer
Gene
Aged
Neoplasm Staging
Regulation of gene expression
Neovascularization
Pathologic
Gene Expression Profiling
General Medicine
Middle Aged
medicine.disease
Up-Regulation
Gene Expression Regulation
Neoplastic
Gene expression profiling
Oncology
030220 oncology & carcinogenesis
Carcinoma
Squamous Cell
Cancer research
Cytokines
Intercellular Signaling Peptides and Proteins
Female
Chemokines
medicine.symptom
Cell Adhesion Molecules
Peptide Hydrolases
Transcription Factors
Zdroj: Tumori Journal. 97:86-94
ISSN: 2038-2529
0300-8916
DOI: 10.1177/030089161109700116
Popis: Aims and backgroundAngiogenesis is a key process in the early stages of tumor development. In this study we aimed to evaluate the expression of a panel of angiogenesis-related genes in a group of Bulgarian patients with early-stage non-small cell lung cancer (NSCLC).Methods and study designWe analyzed the expression of 84 genes associated with the angiogenic process in 12 NSCLCs of two histological subtypes: 7 adenocarcinomas and 5 squamous cell carcinomas. Eight peripheral nontumorous tissues were used as controls. We performed real-time PCR on pathway-specific gene arrays (SABiosciences).ResultsOur pilot study identified upregulated genes in early-stage NSCLC including growth factors (TGFA and EFNA3), the adhesion molecule THBS2, cytokines and chemokines (MDK, CXCL9, CXCL10), and the serine protease PLAU. Several genes showed downregulation including one growth factor (FIGF), the receptors for growth factors TEK and S1PR1 as well as adhesion molecules (COL4A3 and CDH5), the cytokine IL6, the matrix protein LEP and the transcription factor NOTCH4. The study demonstrated deregulated genes specific for the two histological subtypes including the transcription factor HAND2, which was overexpressed in squamous cell carcinomas but not adenocarcinomas.ConclusionsDespite the limited number of patients, our results demonstrated the potential of angiogenesis-related genes as biomarkers in the early stages of NSCLC development. Free full text available at www.tumorionline.it
Databáze: OpenAIRE
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