Effect of Detergents and Osmolytes on Thermal Stability of Native and Mutant Rhodobacter sphaeroides Reaction Centers
| Autor: | L. G. Vasilieva, T. Y. Fufina |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Photosynthetic reaction centre
Sucrose Hot Temperature Protein Conformation Detergents Photosynthetic Reaction Center Complex Proteins Mutation Missense Rhodobacter sphaeroides macromolecular substances Biochemistry Purple bacteria chemistry.chemical_compound Sodium Cholate Maltose biology Osmolar Concentration Trehalose General Medicine biology.organism_classification Membrane Amino Acid Substitution chemistry Osmolyte Biophysics |
| Zdroj: | Biochemistry (Moscow). 86:517-524 |
| ISSN: | 1608-3040 0006-2979 |
| Popis: | Photosynthetic reaction center (RC) of the purple bacterium Rhodobacter sphaeroides is one of the most well-studied transmembrane pigment-protein complexes. It is a relatively stable protein with established conditions for its isolation from membranes, purification, and storage. However, it has been shown that some amino acid substitutions can affect stability of the RC, which results in a decrease of the RCs yield during its isolation and purification, disturbs spectral properties of the RCs during storage, and can lead to sample heterogeneity. To optimize conditions for studying mutant RCs, the effect of various detergents and osmolytes on thermal stability of the complex was examined. It was shown that trehalose and, to a lesser extent, sucrose, maltose, and hydroxyectoin at 1 M concentration slow down thermal denaturation of RCs. Sodium cholate was found to have significant stabilizing effect on the structure of native and genetically modified RCs. The use of sodium cholate as a detergent has several advantages and can be recommended for the storage and investigation of the unstable mutant membrane complexes of purple bacteria in long-term experiments. |
| Databáze: | OpenAIRE |
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