Confirmation of Chlamydophila abortus in infected cell culture using Indirect Immunofluorescence technique
| Autor: | Sreeja R. Nair, M. Mini, V. Jayaprakasan, G. K. Nair |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Indirect immunofluorescence
General Veterinary medicine.diagnostic_test medicine.drug_class Chlamydophila abortus Veterinary medicine Biology Isolation (microbiology) Immunofluorescence biology.organism_classification Monoclonal antibody Virology SF1-1100 McCoy cell lines Serology Animal culture Antigen Cell culture SF600-1100 medicine Monoclonal antibodies |
| Zdroj: | Veterinary World, Vol 4, Iss 10.000, Pp 473-474 (2011) |
| ISSN: | 2231-0916 0972-8988 |
| Popis: | Chlamydophila abortus (C. abortus) is an important abortifacient agent in bovines and ovines. Clinical diagnosis of the disease is often difficult. An early diagnosis can be achieved based on direct demonstration of the organism in clinical material and through the cultural recovery of the organism in embryonated chicken egg. For confirmatory diagnosis antigen detection methods or serological techniques can be adopted. The present study is aimed at the confirmatory diagnosis of C. abortus infection by indirect immunofluorescence technique following the isolation of the organism in cell culture. Specific apple green fluorescing inclusions of C. abortus in McCoy cell lines was detected from 72 h to 96 h post infection employing anti-chlamydial group specific monoclonal antibodies. Thus, a confirmatory diagnosis of the infection was possible with this study. [Vet. World 2011; 4(10.000): 473-474] |
| Databáze: | OpenAIRE |
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