The PMAIP1 Gene on Chromosome 18 is a Candidate Tumor Suppressor Gene in Human Pancreatic Cancer
| Autor: | Shinichi Egawa, Lefter Lp, Michiaki Unno, Makoto Sunamura, Toru Furukawa, Masaharu Ishida, Masanori Akada, Rina Morita, Akira Horii |
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| Rok vydání: | 2008 |
| Předmět: |
Tumor suppressor gene
Physiology Transplantation Heterologous Mice Nude Biology Loss of heterozygosity Mice Chromosome 18 Cell Line Tumor Pancreatic cancer medicine Animals Humans Genes Tumor Suppressor Genetic Predisposition to Disease Cell Proliferation Gene knockdown Gene Expression Profiling Gastroenterology Cancer Microarray Analysis medicine.disease Candidate Tumor Suppressor Gene Pancreatic Neoplasms Gene expression profiling Proto-Oncogene Proteins c-bcl-2 Disease Progression Cancer research Female RNA Interference Chromosomes Human Pair 18 |
| Zdroj: | Digestive Diseases and Sciences. 53:2576-2582 |
| ISSN: | 1573-2568 0163-2116 |
| DOI: | 10.1007/s10620-007-0154-1 |
| Popis: | Frequent loss of heterozygosity on the long arm of chromosome 18 is observed in pancreatic cancer. Previous studies suggested the existence of one or more tumor-suppressor genes other than SMAD4 on chromosome 18. To identify the candidate tumor-suppressor gene(s), we compared gene expression by cDNA microarray analyses using a pancreatic cancer cell line Panc-1 and its hybrid cell lines showing suppressed cell growth after introduction of one normal copy of chromosome 18. The microarray analyses identified 38 genes on chromosome 18 that showed differential expressional levels. Among these genes, phorbol-12-myristate-13-acetate-induced protein 1 (PMAIP1/APR/NOXA) was identified as one of the candidates for tumor suppressor. Expression vector-mediated introduction of PMAIP1 suppressed cell proliferation, and RNAi-mediated knockdown of PMAIP1 induced recovery of cell growth. These results suggest that PMAIP1 may play an important role in the progression of pancreatic cancer. |
| Databáze: | OpenAIRE |
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