Isolation and Characterization of a Non Sex-Specific Lipoprotein from Hemolymph of Fresh Water CrabPotamon potamios
| Autor: | George Fragkiadakis, Efterpi Carpeli-Moustaizi, Emmanoel Stratakis |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
Immunodiffusion Brachyura Fresh Water Biochemistry chemistry.chemical_compound High-density lipoprotein Hemolymph Phosphatidylcholine Animals Amino Acids Polyacrylamide gel electrophoresis Gel electrophoresis chemistry.chemical_classification Chromatography Fatty acid Anatomy Isoelectric point chemistry Chromatography Gel Electrophoresis Polyacrylamide Gel Female lipids (amino acids peptides and proteins) Density gradient ultracentrifugation Lipoproteins HDL Lipoprotein |
| Zdroj: | Biological Chemistry Hoppe-Seyler. 373:665-674 |
| ISSN: | 0177-3593 |
| DOI: | 10.1515/bchm3.1992.373.2.665 |
| Popis: | A non sex-specific high density lipoprotein (HDL) was isolated from the hemolymph of male fresh water crab Potamon potamios. Antiserum against male lipoprotein precipitated a lipoprotein from ovigerous and non ovigerous females. The lipoprotein was isolated by a combination of KBr density gradient ultracentrifugation and gel filtration on Bio-Gel A-1.5m. Two yellow bands with hydrated density of 1.08 and 1.10 g x ml-1 were observed following density gradient ultracentrifugation. Native polyacrylamide electrophoresis showed a low- and a high-mobility band, staining with Sudan black. Both native bands were resolved to a single 128 kDa band in SDS-PAGE. The relative molecular masses of the two bands, as determined by gel filtration and native gradient gel electrophoresis, were 230 and 440 kDa, respectively. Since the lipid content was 50% and the molecular mass of the apoprotein was 128 kDa, we suggest that the lipoprotein is a monomer and a dimer. Chemical cross-linking experiments support this observation. The isoelectric point of the lipoprotein was pH 5.8. The amino-acid composition was also determined. The lipoprotein contained 50% lipids, with phospholipids being the predominant species (80%). Phosphatidylcholine (66%) and phosphatidylethanolamine (16%) were the predominant phospholipids. The fatty acid content of the lipoprotein was also determined. The lipoprotein contained 68% unsaturated fatty acids with oleate being the dominant species (35%). |
| Databáze: | OpenAIRE |
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