Acetylcholinesterase inhibitory guided fractionation of Melissa officinalis L
| Autor: | Raimo Hiltunen, Päivi Järvinen, Gustav Boije af Gennäs, Petri Lackman, H.J. Damien Dorman, Velimatti Ollilainen, Jari Yli-Kauhaluoma, Keyvan Dastmalchi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
activity-guided fractionation
Antioxidant medicine.medical_treatment Clinical Biochemistry Pharmaceutical Science Pharmacology Pharmacognosy Depsides Melissa Biochemistry acetylcholinesterase inhibition Antioxidants 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Melissa officinalis Alzheimer Disease Drug Discovery medicine Humans Molecular Biology 030304 developmental biology 0303 health sciences biology Plant Extracts Chemistry Rosmarinic acid cis-rosmarinic acid Organic Chemistry Biological activity Phenolic acid Acetylcholinesterase Kinetics Cinnamates Enzyme inhibitor biology.protein Molecular Medicine Cholinesterase Inhibitors trans-rosmarinic acid 030217 neurology & neurosurgery |
| Zdroj: | Dastmalchi, K, Ollilainen, V, Lackman, P, Boije af Gennäs, G, Dorman, H J D, Järvinen, P P, Yli-Kauhaluoma, J & Hiltunen, R 2009, ' Acetylcholinesterase inhibitory guided fractionation of Melissa officinalis L. ', Bioorganic & Medicinal Chemistry, vol. 17, no. 2, pp. 867-871 . https://doi.org/10.1016/j.bmc.2008.11.034 |
| DOI: | 10.1016/j.bmc.2008.11.034 |
| Popis: | The plant Melissa officinalis L. has been used traditionally in the treatment of cognitive dysfunction. Based on its traditional medicinal use, it was assessed for its clinical efficacy in mild to moderate Alzheimer’s patients. The plant was effective in the management of the disease. Therefore, based on this result, a similar plant extract was prepared in order to be screened for bioactivities which are relevant in Alzheimer’s disease therapy. The extract was recently screened for antioxidant activity and it showed a wide range of antioxidant properties. Another important bioactivity is acetylcholinesterase inhibition, which the extract was screened for in the current investigation. The extract was capable of inhibiting the enzyme in a time and dose-dependent manner. Activity of the extract at 10 min was estimated as 1.72 ± 0.16 μg equivalents of physostigmine/mg of the extract. Acetylcholinesterase inhibitory guided fractionation of the extract was then carried out. Most of the fractions showed inhibitory activity and were more potent than the extract. The contents of the most potent fraction were identified as cis- and trans-rosmarinic acid isomers and a rosmarinic acid derivative using LC-DAD-ESI-MS and NMR methods. |
| Databáze: | OpenAIRE |
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