Free fatty acids modulate LDL receptor activity in BHK-21 cells
| Autor: | Maurizio Bifulco, Daniela Lattero, Paolo Mondola, Rosalba Vitelli, Tiziana Annella, Rosalba Serù, Cecilia Bucci, Mariarosaria Santillo |
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| Přispěvatelé: | Bucci, Cecilia, Serù, R, Annella, T, Vitelli, R, Lattero, D, Bifulco, M, Mondola, P, Santillo, M., Bucci, C., Seru, R., Annella, T., Vitelli, R., Lattero, D., Bifulco, M., Mondola, Paolo, Santillo, Mariarosaria |
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Linoleic acid Blotting Western Biology Fatty Acids Nonesterified Kidney Cell Line Fatty Acids Monounsaturated chemistry.chemical_compound Internal medicine Cricetinae medicine Animals Lovastatin Cells Cultured chemistry.chemical_classification L-Lactate Dehydrogenase Fatty acid Fibroblasts Elaidic acid Hydroxycholesterols Oleic acid Endocrinology chemistry Biochemistry Receptors LDL LDL receptor Free fatty acid receptor Fatty Acids Unsaturated Arachidonic acid lipids (amino acids peptides and proteins) Hydroxymethylglutaryl-CoA Reductase Inhibitors Cardiology and Cardiovascular Medicine Polyunsaturated fatty acid |
| Zdroj: | Atherosclerosis. 137(2) |
| ISSN: | 0021-9150 |
| Popis: | It has been shown that dietary fatty acids affect serum low density lipoprotein (LDL) levels, but the mechanism responsible for this effect is still under debate. Here we investigate the effect of different free fatty acids on LDL receptor activity in BHK-21 cells. These cells possess a classical LDL receptor strongly regulated by substances like 25-OH-cholesterol or lovastatin. Preincubation of cells for 24 h with both oleic (cis 18:1) and its trans counterpart, elaidic acid, enhanced I-125-LDL binding, internalization and degradation, being oleic acid more effective than elaidic acid. Among polyunsaturated fatty acids (PUFA) of the n-6 series arachidonic acid (20:4) enhanced LDL receptor activity more than linoleic acid (18:2), and among PUFA of the n-3 series docosahexaenoic (22:6) and eicosapentaenoic acids (20:5) were more effective compared to a-linolenic acid (18:3). Conversely, preincubation of cells with saturated fatty acids, palmitic (16:0) and stearic (18:0) acids, decreased binding, internalization and degradation of I-125-LDL. Scatchard analysis of binding data obtained with palmitic and oleic acids showed that these two fatty acids affect LDL receptor number without altering receptor affinity. The regulatory effect of free fatty acids on LDL receptor activity in BHK-21 cells is consistent with the hypothesis that the ability of fatty acids to modulate LDL-cholesterol levels in vivo is mediated, at least in part, by an action on receptor-dependent uptake of LDL. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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