A high molecular weight component of the human tumor necrosis factor receptor is associated with cytotoxicity
| Autor: | Abla A. Creasey, Charles R. Vitt, Ralph Yamamoto |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Cell Survival
Receptors Cell Surface Receptors Tumor Necrosis Factor Cell Line law.invention Recombinant tumor necrosis factor law Humans Cytotoxic T cell Receptor Cytotoxicity Glycoproteins chemistry.chemical_classification Multidisciplinary Tumor Necrosis Factor-alpha Growth Inhibitors Recombinant Proteins Molecular Weight Kinetics chemistry Biochemistry Cell culture Recombinant DNA Tumor necrosis factor alpha Glycoprotein Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 84:3293-3297 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.84.10.3293 |
| Popis: | We compared the molecular structure of the receptor to human recombinant tumor necrosis factor (HurTNF) on cells of different tissue origin that differ in their response to one of the known activities of TNF. We studied tumor cell lines that respond to the cytotoxic action of TNF and resistant variants that bind TNF, normal cell lines that are stimulated to proliferate by TNF and those that are not affected by TNF, and peripheral blood granulocytes whose activation is also augmented by TNF. Using 125I-labeled HurTNF, we found that it bound mainly to four cellular polypeptides (138, 90, 75, and 54 kDa), three of which were found in every cell type examined and one (138 kDa) that was observed only in a human breast carcinoma cell line (MCF-7) that is highly responsive to the cytotoxic action of TNF. The 138-kDa polypeptide was not found in resistant variants of MCF-7 that bind TNF. In contrast to the other polypeptides, the 138-kDa protein was detected 30 min after incubation at 4 degrees C, as compared to 5 min. Scatchard analysis and cross-linking data suggest a model for the TNF receptor structure whereby the receptor is composed of noncovalently linked membrane-bound polypeptides that bind TNF with high affinity (Kd, 0.05-0.8 X 10(-9) M) with the 138-kDa protein being the least abundant and/or even absent in most cells. |
| Databáze: | OpenAIRE |
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