Long non-coding RNA AC245100.4 promotes prostate cancer tumorigenesis via the microRNA-145-5p/RBBP5 axis
| Autor: | Ping Lin, Jiahui Wan, Xu Yang, Hui Xie, Weiyu Yang, Qianqian Wang, Xiaoguang Yu, Jianing Zhao, Jiabin Zhao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Cancer Research Carcinogenesis retinoblastoma binding protein 5 Cell Biology medicine.disease_cause 03 medical and health sciences Mice 0302 clinical medicine Cell Movement Cell Line Tumor microRNA medicine Gene silencing Animals Humans competing endogenous RNA Cell Proliferation Gene knockdown Competing endogenous RNA long non-coding RNA AC245100.4 microRNA-145-5p Computational Biology Prostatic Neoplasms General Medicine Articles Cell cycle prostate cancer Xenograft Model Antitumor Assays Long non-coding RNA Up-Regulation DNA-Binding Proteins Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Gene Knockdown Techniques Cancer research RNA Long Noncoding |
| Zdroj: | Oncology Reports |
| ISSN: | 1791-2431 1021-335X |
| Popis: | Long non‑coding RNAs (lncRNAs) are markedly involved in cancer progression. Thus, identification of these lncRNAs can aid in the treatment of cancer. The present study focused on investigating the overall biological function, mechanism of action and clinical importance of lncRNA AC245100.4 in prostate cancer (PCa). The present study identified that AC245100.4 expression was significantly upregulated in PCa tissues and cell lines. Knockdown of AC245100.4 impaired tumor growth in an animal model. Biological function analysis indicated that AC245100.4 overexpression notably promoted cell proliferation and migration, while knockdown of AC245100.4 suppressed cell proliferation and migration. Mechanism studies focused on the competing endogenous RNA (ceRNA) network of AC245100.4. Bioinformatics predictions indicated that both AC245100.4 and retinoblastoma binding protein 5 (RBBP5) had microRNA (miR) response elements for miR‑145‑5p. This was further verified using a dual luciferase and RNA immunoprecipitation assays. AC245100.4 could positively regulate RBBP5 expression, but negatively regulated miR‑145‑5p expression. In addition, AC245100.4 knockdown‑mediated inhibitory effects on cell proliferation and migration could be reversed by miR‑145‑5p silencing. Overall, the present study proposed a novel model in which the AC245100.4/miR‑145‑5p/RBBP5 ceRNA network induced the development of PCa, providing novel insights for PCa treatment. |
| Databáze: | OpenAIRE |
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