Over-expression of CarMT gene modulates the physiological performance and antioxidant defense system to provide tolerance against drought stress in Arabidopsis thaliana L
| Autor: | Mohd Akram Ansari, Anil Kumar, Vivek Pandey, Arvind Kumar Dubey, Shekhar Mallick, Ambedkar Gautam, Nayan Sahu, Navin Kumar, Indraneel Sanyal, Ruma Ranjan, Veena Pande, Meenakshi, Soumit K. Behera |
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| Rok vydání: | 2018 |
| Předmět: |
Proline
Health Toxicology and Mutagenesis Transgene 0211 other engineering and technologies Arabidopsis Germination 02 engineering and technology 010501 environmental sciences 01 natural sciences Antioxidants Gene Expression Regulation Plant Stress Physiological Metallothionein Arabidopsis thaliana Cysteine Gene 0105 earth and related environmental sciences Plant Proteins 021110 strategic defence & security studies biology fungi Public Health Environmental and Occupational Health food and beverages General Medicine biology.organism_classification APX Plants Genetically Modified Pollution Adaptation Physiological Cicer Cell biology Droughts Silique |
| Zdroj: | Ecotoxicology and environmental safety. 171 |
| ISSN: | 1090-2414 |
| Popis: | Drought is one of the major abiotic stresses which negatively affect plant growth and crop yield. Metallothionein (MTs) is a low molecular weight protein, mainly involved in metal homeostasis, while, its role in drought stress is still to be largely explored. The present study was aimed to investigate the role of MT gene against drought stress. The chickpea MT based on its up-regulation under drought stress was overexpressed in Arabidopsis thaliana to explore its role in mitigation of drought stress. The total transcript of MT gene was up to 30 fold higher in transgenic lines. Arabidopsis plants transformed with MT gene showed longer roots, better efficiency of survival and germination, larger siliques and higher biomass compared to WT. The physiological variables (A, WUE, G, E, qP and ETR) of WT plants were reduced during drought stress which recovered in transgenic Arabidopsis lines. The enzymatic and non-enzymatic antioxidant (APX, GPX, POD, GR, GRX, GST, CAT, MDHAR, ASc and GSH) levels were also enhanced in transgenic lines to provide tolerance. Simultaneously, drought responsive amino acids, i.e. proline and cysteine contents were higher in transgenic lines. Overall, the results suggest that MT gene is actively involved in the mitigation of drought stress and could be the choice for genetic engineering strategy to overcome drought stress. |
| Databáze: | OpenAIRE |
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