Galactosylated PVDF membrane promotes hepatocyte attachment and functional maintenance
| Autor: | Peng-Chi Zhang, Ser-Mien Chia, Jun Wang, Hanry Yu, Zhi-Qun Tang, Wei Seng Lim, Hai-Quan Mao, Kam W. Leong, Hong-Fang Lu |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Materials science Biophysics Bioengineering Biomaterials chemistry.chemical_compound Adsorption Coated Materials Biocompatible Cytochrome P-450 Enzyme System Albumins Materials Testing Polymer chemistry Cell Adhesion medicine Animals Rats Wistar Cells Cultured Cell Size Polypropylene chemistry.chemical_classification Tissue Engineering Spheroid Galactose Substrate (chemistry) Membranes Artificial Polymer Poloxamer Liver Artificial Rats medicine.anatomical_structure Membrane chemistry Mechanics of Materials Hepatocyte Hepatocytes Ceramics and Composites Polyvinyls Cell Division |
| Zdroj: | Biomaterials. 24:4893-4903 |
| ISSN: | 0142-9612 |
| Popis: | One of the major challenges in BLAD design is to develop functional substrates suitable for hepatocyte attachment and functional maintenance. In the present study, we designed a poly(vinylidene difluoride) (PVDF) surface coated with galactose-tethered Pluronic polymer. The galactose-derived Pluronic F68 (F68-Gal) was adsorbed on PVDF membrane through hydrophobic-hydrophobic interaction between PVDF and the polypropylene oxide segment in Pluronic. The galactose density on the modified PVDF surface increased with the concentration of the F68-Gal solution, reaching 15.4 nmol galactosyl groups per cm2 when a 1 mg/ml of F68-Gal solution was used. The adsorbed F68-Gal remained relatively stable in culture medium. Rat hepatocytes attachment efficiency on F68-Gal modified PVDF membrane was similar to that on collagen-coated surface. The attached hepatocytes on PVDF/F68-Gal membrane self-assembled into multi-cellular spheroids after 1 day of culture. These attached hepatocytes in spheroids exhibited higher cell functions such as albumin synthesis and P450 1A1 detoxification function compared to unmodified PVDF membrane and collagen-coated surface. These results suggest the potential of this galactose-immobilized PVDF membrane as a suitable substrate for hepatocyte culture. |
| Databáze: | OpenAIRE |
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