CELLULAR AUTOPHAGOCYTOSIS INDUCED BY X-IRRADIATION AND VINBLASTINE
| Autor: | Hans Hamberg |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Acid Phosphatase
Vacuole Biology Vinblastine Cell Line law.invention symbols.namesake chemistry.chemical_compound Phagocytosis Immunology and Microbiology (miscellaneous) law Autophagy Humans Thorium dioxide General Immunology and Microbiology Endoplasmic reticulum Acid phosphatase General Medicine Golgi apparatus Endocytosis Cell biology Membrane chemistry Ferritins Vacuoles symbols biology.protein Thorium Dioxide Gold Electron microscope Lysosomes Neuroglia |
| Zdroj: | Acta Pathologica Microbiologica Scandinavica Series A :Pathology. :317-327 |
| ISSN: | 0108-0164 |
| DOI: | 10.1111/j.1699-0463.1983.tb02762.x |
| Popis: | Autophagocytosis was induced in cultured, human glial cells by X-irradiation or exposure to vinblastine sulphate. A transmission electron microscopic investigation of the origin of the segregating membranes in the autophagic process was performed by labelling of endocytotic vacuoles and lysosomes with electron-dense marker particles (native and cationized ferritin, colloidal gold and thorium dioxide). Cytochemical demonstration of the lysosomal marker enzyme acid phosphatase and serial sectioning of the cells were also carried out. The majority of newly formed, double-membrane bounded autophagic vacuoles were devoid of markers for both lysosomes and endocytotic vacuoles. Moreover, no evidence of origin from the endoplasmic reticulum was found and the segregating membranes of this type of autophagic vacuoles were, by process of elimination, considered likely to be derived from Golgi vacuoles or, possibly, assembled de novo. Autophagy also appeared to be effected through an alternative pathway involving a lysosomal wrapping or microautophagic mechanism. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|