Electrochemical Detection of Fluoroquinolone Antibiotics in Milk Using a Magneto Immunosensor

Autor: Francisco Sánchez-Baeza, Maria-Pilar Marco, Daniel G. Pinacho, María Isabel Pividori
Přispěvatelé: Ministerio de Ciencia e Innovación (España), European Commission, Generalitat de Catalunya, Instituto de Salud Carlos III, Fundación EROSKI
Rok vydání: 2014
Předmět:
electrochemical immunosensor
Conductometry
medicine.drug_class
Antibiotics
Food Contamination
Biosensing Techniques
02 engineering and technology
lcsh:Chemical technology
Immunomagnetic separation
01 natural sciences
Biochemistry
Article
magnetic beads
Analytical Chemistry
Matrix (chemical analysis)
antimicrobial residues
medicine
Animals
lcsh:TP1-1185
Electrical and Electronic Engineering
Instrumentation
Immunoassay
milk
Chromatography
medicine.diagnostic_test
Immunomagnetic Separation
Chemistry
010401 analytical chemistry
Extraction (chemistry)
fluoroquinolone antibiotics
food safety
Equipment Design
equipment and supplies
021001 nanoscience & nanotechnology
Atomic and Molecular Physics
and Optics
Amperometry
Anti-Bacterial Agents
3. Good health
0104 chemical sciences
Equipment Failure Analysis
Electrode
0210 nano-technology
human activities
Food Analysis
Fluoroquinolones
Zdroj: Recercat. Dipósit de la Recerca de Catalunya
instname
Sensors; Volume 14; Issue 9; Pages: 15965-15980
Digital.CSIC. Repositorio Institucional del CSIC
Sensors (Basel, Switzerland)
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
Recercat: Dipósit de la Recerca de Catalunya
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Sensors, Vol 14, Iss 9, Pp 15965-15980 (2014)
ISSN: 1424-8220
2008-0557
Popis: An amperometric magneto-immunosensor (AMIS) for the detection of residues of fluoroquinolone antibiotics in milk samples is described for the first time. The immunosensor presented combines magnetic beads biomodified with an antibody with a broad recognition profile of fluoroquinolones, a haptenized enzyme and a magnetic graphite-epoxy composite (m-GEC) electrode. After the immunochemical reaction with specific enzyme tracer, the antibody biomodified magnetic beads are easily captured by an electrode made of graphite-epoxy composite containing a magnet, which also acts as transducer for the electrochemical detection. In spite of the complexity of milk, the use of magnetic beads allows elimination of potential interferences caused by the matrix components; hence the AMIS could perform quantitative measurements, directly in these samples, without any additional sample cleanup or extraction step. The immunosensor is able to detect up to seven different fluoroquinolones far below the MRLs defined by the UE for milk; for example ciprofloxacin is detected directly in milk with an IC50 of 0.74 μg/L and a LOD of 0.009 μg/L. This strategy offers great promise for rapid, simple, cost-effective, and on-site analysis fluoroquinolones in complex samples. © 2014 by the authors; licensee MDPI, Basel, Switzerland.
This work has been supported by the Ministry of Science and Innovation (Spain) (Contract number AGL2008-05578-C05-01 and by the European Community (FP7-FOOD-2007-211326 and FP7-OCEAN-2013-1-614168). The Nb4D group is a consolidated Grup de Recerca de la Generalitat de Catalunya and has support from the Departament d’Universitats, Recerca i Societat de la Informació la Generalitat de Catalunya (expedient 2014 SGR 1484). CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008–2011, IniciativaIngenio 2010, Consolider Program, CIBER Actions and is financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Foundation. DG had a fellowship from the Fundación EROSKI.
Databáze: OpenAIRE
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