Genoprotective and neuroprotective effects ofDaphne gnidiumleaf methanol extract, tested on male mice
| Autor: | Kamel Ghedira, Fadwa Chaabane, Aicha Sassi, Raja Majouli, Imen Mokdad-Bzeouich, Leila Chekir-Ghedira, Nadia Mustapha |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Antioxidant DPPH Health Toxicology and Mutagenesis medicine.medical_treatment Intraperitoneal injection Kidney Toxicology Lethal Dose 50 Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Picrates Toxicity Tests Acute medicine Animals IC50 Butyrylcholinesterase Pharmacology Chemical Health and Safety Dose-Response Relationship Drug Traditional medicine biology Plant Extracts Methanol Biphenyl Compounds Public Health Environmental and Occupational Health Antimutagenic Agents General Medicine Methyl Methanesulfonate biology.organism_classification Methyl methanesulfonate Plant Leaves Comet assay Neuroprotective Agents chemistry Daphne gnidium 030220 oncology & carcinogenesis Daphne Comet Assay Lipid Peroxidation Injections Intraperitoneal 030217 neurology & neurosurgery DNA Damage |
| Zdroj: | Drug and Chemical Toxicology. 39:297-302 |
| ISSN: | 1525-6014 0148-0545 |
| Popis: | Methanol extract of Daphne gnidium leaves was assessed for its antigenotoxic and neuroprotective effects through antioxidant and antibutyrylcholinesterase activities. Antigenotoxic activity was evaluated against methyl methanesulfonate injected intraperitoneally to mice, using the comet assay. The protective effect of D. gnidium reached 99.12%, at the lowest tested dose (44 mg/kg b.w.) in kidney cells, and 92.16% at the dose of 88 mg/kg b.w. in blood cells. The extract was dissolved in water and administrated to mice by intraperitoneal injection. Antioxidant activity was tested against DPPH radicals. It reached a maximum of 74.52% with an IC50 value of 45 µg/ml. Anticholinesterase activity was determined against butyrylcholinesterase, an enzyme linked to Alzheimer disease. The extract exhibited antibutyrylcholinestrase effect with an inhibition percentage of 35.82% at the lowest tested dose (44 mg/kg b.w.). |
| Databáze: | OpenAIRE |
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