Luminescent surfaces with tailored angular emission for compact dark-field imaging devices
| Autor: | Peter T. C. So, Moungi G. Bawendi, Sara Nagelberg, Christopher J. Rowlands, Kurt Broderick, Cécile A. C. Chazot, Yunjo Kim, Mathias Kolle, Igor Coropceanu, Maik R. J. Scherer |
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| Rok vydání: | 2020 |
| Předmět: |
Microscope
Materials science ILLUMINATION 02 engineering and technology Substrate (printing) SUBSTEPS 01 natural sciences Article Physics Applied law.invention 010309 optics Optical microscope law 0103 physical sciences Microscopy 01 Mathematical Sciences Science & Technology 02 Physical Sciences business.industry Physics Optics MICROSCOPY 021001 nanoscience & nanotechnology Dark field microscopy Atomic and Molecular Physics and Optics Optoelectronics & Photonics Electronic Optical and Magnetic Materials PRECISION Physical Sciences Optoelectronics Spatial frequency Photonics 0210 nano-technology Luminescence business |
| Zdroj: | Nature photonics |
| ISSN: | 1749-4893 1749-4885 |
| DOI: | 10.1038/s41566-020-0593-1 |
| Popis: | Dark-field microscopy is a standard imaging technique widely employed in biology that provides high image contrast for a broad range of unstained specimens1. Unlike bright-field microscopy, it accentuates high spatial frequencies and can therefore be used to emphasize and resolve small features. However, the use of dark-field microscopy for reliable analysis of blood cells, bacteria, algae and other marine organisms often requires specialized, bulky microscope systems, as well as expensive additional components, such as dark-field-compatible objectives or condensers2,3. Here, we propose to simplify and downsize dark-field microscopy equipment by generating the high-angle illumination cone required for dark-field microscopy directly within the sample substrate. We introduce a luminescent photonic substrate with a controlled angular emission profile and demonstrate its ability to generate high-contrast dark-field images of micrometre-sized living organisms using standard optical microscopy equipment. This new type of substrate forms the basis for miniaturized lab-on-chip dark-field imaging devices that are compatible with simple and compact light microscopes. A luminescent photonic substrate with a controlled angular emission profile is introduced and its ability to generate high-contrast dark-field images of micrometre-sized living organisms is demonstrated using standard optical microscopy equipment. |
| Databáze: | OpenAIRE |
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