Expansion of Necrosis Depending on Hybrid Motor-Driven Motility of Aeromonas hydrophila in a Murine Wound Infection Model
| Autor: | Takashige Kashimoto, Moeko Yamasaki, Mayu Nomura, Kohei Yamazaki, Shunji Ueno, Ayuha Niwano, Yukihiro Akeda |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Aeromonas hydrophila Necrosis Fulminant Motility Flagellum Microbiology flagellum 03 medical and health sciences Virology necrotic soft tissue infection medicine Pathogen lcsh:QH301-705.5 030304 developmental biology 0303 health sciences biology Strain (chemistry) 030306 microbiology biology.organism_classification motility lcsh:Biology (General) medicine.symptom Bacteria |
| Zdroj: | Microorganisms, Vol 9, Iss 10, p 10 (2021) Microorganisms Volume 9 Issue 1 |
| ISSN: | 2076-2607 |
| Popis: | The gram-negative bacterium Aeromonas hydrophila is a cause of fulminant and lethal necrotizing soft tissue infections (NSTIs). Suppressing the rapid proliferation of the pathogen and expansion of the necrosis caused in the host is an important issue in clinical practice, but the pathogenic mechanism for the rapid aggravation has not been clarified. In this study, we characterized the function of two types of motor stators in A. hydrophila and explored the role of motility during wound infection. In vitro analysis showed that the motility was reliably maintained while being complemented by the stators. We created a non-motile strain that lacked genes encoding two types of motor stators and analyzed the role of motility in a murine wound infection model. Examination of the bacterial burden in the local infection site and systemic circulation revealed that motility was not essential for the proliferation of A. hydrophila in the host. However, the extent of necrosis at the lesions was lower, and survival times were prolonged in mice infected with the non-motile strain compared with mice infected with the parent strain. These results provide evidence that the rapid expansion of necrosis and the progression to death within a short time period is dependent on the motility of A. hydrophila. |
| Databáze: | OpenAIRE |
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