Detection and Genome Sequencing of SARS-CoV-2 in a Domestic Cat with Respiratory Signs in Switzerland

Autor: Klaus, Julia, Meli, Marina L, Willi, Barbara, Nadeau, Sarah, Beisel, Christian, Stadler, Tanja, Egberink, Herman, Zhao, Shan, Lutz, Hans, Riond, Barbara, Rösinger, Nina, Stalder, Hanspeter, Renzullo, Sandra, Hofmann-Lehmann, Regina, Klinische infectiologie en microb. lab., Virologie, dI&I I&I-1, dI&I I&I-4
Přispěvatelé: University of Zurich, Klinische infectiologie en microb. lab., Virologie, dI&I I&I-1, dI&I I&I-4
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Male
10253 Department of Small Animals
Neutralizing activity
Human-to-feline transmission
viruses
lcsh:QR1-502
medicine.disease_cause
Cat Diseases
lcsh:Microbiology
Serology
Feces
One-Health
11434 Center for Clinical Studies
610 Medicine & health
Phylogeny
Coronavirus
CATS
630 Agriculture
Companion animals
Infectious Diseases
Nasal Swab
11404 Department of Clinical Diagnostics and Services
RNA
Viral
medicine.symptom
Switzerland
030106 microbiology
Context (language use)
Genome
Viral
Biology
Asymptomatic
Polymorphism
Single Nucleotide
Article
03 medical and health sciences
Contamination
Next generation sequencing
Virology
medicine
Animals
SARS-CoV-2
Domestic cat
COVID-19
2725 Infectious Diseases
domestic cat
companion animals
next generation sequencing
serology
neutralizing activity
human-to-feline transmission
contamination
medicine.disease
030104 developmental biology
Upper respiratory tract infection
2406 Virology
Cats
570 Life sciences
biology
Zdroj: Klaus, Julia; Meli, Marina L; Willi, Barbara; Nadeau, Sarah; Beisel, Christian; Stadler, Tanja; Sars-CoV-Sequencing Team, ETH; Egberink, Herman; Zhao, Shan; Lutz, Hans; Riond, Barbara; Rösinger, Nina; Stalder, Hanspeter; Renzullo, Sandra; Hofmann-Lehmann, Regina (2021). Detection and Genome Sequencing of SARS-CoV-2 in a Domestic Cat with Respiratory Signs in Switzerland. Viruses, 13(3) Molecular Diversity Preservation International MDPI 10.3390/v13030496
Viruses, 13(3), 1. Multidisciplinary Digital Publishing Institute (MDPI)
Viruses
Viruses, 13 (3)
Volume 13
Issue 3
Viruses, Vol 13, Iss 496, p 496 (2021)
ISSN: 1999-4915
DOI: 10.3390/v13030496
Popis: Since the emergence of coronavirus disease (COVID-19) in late 2019, domestic cats have been demonstrated to be susceptible to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) under natural and experimental conditions. As pet cats often live in very close contact with their owners, it is essential to investigate SARS-CoV-2 infections in cats in a One-Health context. This study reports the first SARS-CoV-2 infection in a cat in a COVID-19-affected household in Switzerland. The cat (Cat 1) demonstrated signs of an upper respiratory tract infection, including sneezing, inappetence, and apathy, while the cohabiting cat (Cat 2) remained asymptomatic. Nasal, oral, fecal, fur, and environmental swab samples were collected twice from both cats and analyzed by RT-qPCR for the presence of SARS-CoV-2 viral RNA. Both nasal swabs from Cat 1 tested positive. In addition, the first oral swab from Cat 2 and fur and bedding swabs from both cats were RT-qPCR positive. The fecal swabs tested negative. The infection of Cat 1 was confirmed by positive SARS-CoV-2 S1 receptor binding domain (RBD) antibody testing and neutralizing activity in a surrogate assay. The viral genome sequence from Cat 1, obtained by next generation sequencing, showed the closest relation to a human sequence from the B.1.1.39 lineage, with one single nucleotide polymorphism (SNP) difference. This study demonstrates not only SARS-CoV-2 infection of a cat from a COVID-19-affected household but also contamination of the cats' fur and bed with viral RNA. Our results are important to create awareness that SARS-CoV-2 infected people should observe hygienic measures to avoid infection and contamination of animal cohabitants.
Viruses, 13 (3)
ISSN:1999-4915
Databáze: OpenAIRE
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