Tumor Targeting Effect of Triphenylphosphonium Cations and Folic Acid Coated with Zr-89-Labeled Silica Nanoparticles
| Autor: | Sang Wook Kim, Jeong Hoon Park, Jun Young Lee, Pyeong Seok Choi, Gun Gyun Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cell
PET imaging Pharmaceutical Science Nanoparticle 02 engineering and technology silica nanoparticles Analytical Chemistry Mice Drug Discovery Receptor Internalization media_common Membrane potential Mice Inbred BALB C 0303 health sciences Chemistry Silicon Dioxide 021001 nanoscience & nanotechnology medicine.anatomical_structure Chemistry (miscellaneous) Colonic Neoplasms Molecular Medicine Female Zr-89 0210 nano-technology Drug carrier media_common.quotation_subject Conjugated system Article lcsh:QD241-441 03 medical and health sciences folic acid Organophosphorus Compounds lcsh:Organic chemistry Cations medicine Animals Humans Physical and Theoretical Chemistry 030304 developmental biology Radioisotopes Organic Chemistry Positron-Emission Tomography Cancer cell Nanoparticles Zirconium Radiopharmaceuticals triphenylphosphonium cation surface modification Nuclear chemistry |
| Zdroj: | Molecules, Vol 25, Iss 2922, p 2922 (2020) Molecules Volume 25 Issue 12 |
| ISSN: | 1420-3049 |
| Popis: | In this study, we investigated the tumor targeting effect in cancer cells using triphenylphosphonium (TPP) cations, which are accumulated by differences in membrane potential, and folic acid (FA), which is selectively bound to overexpressed receptors on various cancer cells. We used Food and Drug Administration (FDA)-approved silica nanoparticles (SNPs) as drug carriers, and SNPs conjugated with TPP and FA (STFs) samples were prepared by introducing different amounts of TPP and FA onto the nanoparticle surfaces. STF-1, 2, 3, 4 and 5 are named according to the combination ratio of TPP and FA on the particle surface. To confirm the tumor targeting effect, 89Zr (t1/2 = 3.3 days) was coordinated directly to the silanol group of SNP surfaces without chelators. It was shown that the radiochemical yield was 69% and radiochemical purity was > 99%. In the cellular uptake evaluation, SNPs with the most TPP (SFT-5) and FA (SFT-1) attached indicated similar uptake tendencies for mouse colon cancer cells (CT-26). However, the results of the cell internalization assay and measurement of positron emission tomography (PET) images showed that SFT-5 had more affinity for the CT-26 tumor than other samples the TPP ratio of which was lower. Consequently, we confirmed that TPP ligands affect target cancer cells more than FA, which means that cell membrane potential is significantly effective for tumor targeting. |
| Databáze: | OpenAIRE |
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