Rapid detection of methicillin-resistant Staphylococcus aureus in screening samples by relative quantification between the mecA gene and the SA442 gene
| Autor: | Norbert Vaessen, Paul H. M. Savelkoul, Perry van Wunnik, Lonneke G.M. Bode, Leo C. Smeets |
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| Přispěvatelé: | Medische Microbiologie, RS: NUTRIM - R3 - Chronic inflammatory disease and wasting, RS: CAPHRI School for Public Health and Primary Care, Medical Microbiology & Infectious Diseases, Medical Microbiology and Infection Prevention, CCA - Immuno-pathogenesis |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Microbiology (medical) Penicillin binding proteins Biology medicine.disease_cause Staphylococcal infections Polymerase Chain Reaction Microbiology law.invention Bacterial Proteins law medicine Humans Mass Screening Penicillin-Binding Proteins Molecular Biology Polymerase chain reaction Mass screening Bacteriological Techniques SCCmec Staphylococcal Infections biochemical phenomena metabolism and nutrition medicine.disease bacterial infections and mycoses Virology Methicillin-resistant Staphylococcus aureus Molecular Diagnostic Techniques Genes Bacterial Staphylococcus aureus Carrier State Staphylococcus |
| Zdroj: | Journal of Microbiological Methods, 89(2), 129-132. Elsevier Bode, L G M, van Wunnik, P, Vaessen, N, Savelkoul, P H M & Smeets, L 2012, ' Rapid detection of methicillin-resistant Staphylococcus aureus in screening samples by relative quantification between the mecA gene and the SA442 gene ', Journal of Microbiological Methods, vol. 89, no. 2, pp. 129-132 . https://doi.org/10.1016/j.mimet.2012.02.014 |
| ISSN: | 1872-8359 0167-7012 |
| DOI: | 10.1016/j.mimet.2012.02.014 |
| Popis: | Rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) is important to identify patients colonized with this pathogen and implement infection control precautions. We aimed to improve the combined use of the mecA gene polymerase chain reaction (PCR) and the SA442 PCR to detect MRSA in clinical screening samples. In a true MRSA the mecA copy number will be equal to the SA442 copy number, whereas in samples with a methicillin-sensitive Staphylococcus aureus (MSSA) combined with a methicillin-resistant coagulase-negative Staphylococcus (MRCNS) the copy numbers will usually differ. Here we introduce a PCR system, relative quantification PCR (RQ-PCR), which takes this difference into account. RQ-PCR identifies true MRSA in clinical samples with a specificity that is comparable to the SCCmec-based PCRs. (C) 2012 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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